Understanding the anomalous electrophoresis of bent DNA molecules: a reptation model

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Science  28 Jul 1989:
Vol. 245, Issue 4916, pp. 396-399
DOI: 10.1126/science.2756426


In polyacrylamide gel electrophoresis, the retardation of DNA molecules containing regions of intrinsic curvature can be explained by a novel reptation model that includes the elastic free energy of the DNA chain. Computer simulations based on this model give results that reproduce the dependence of anomalous mobility on gel concentration, which is quantified by new experimental data on the mobilities of circularly permuted isomers of kinetoplast DNA fragments. Fitting of the data required allowing for the elasticity of the gel.

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