The location of DNA in RecA-DNA helical filaments

See allHide authors and affiliations

Science  28 Jul 1989:
Vol. 245, Issue 4916, pp. 404-407
DOI: 10.1126/science.2667137


The helical filament that the RecA protein of Escherichia coli forms around DNA is the active apparatus in protein-catalyzed homologous genetic recombination. The actual position of DNA within this complex has been unknown. Image analysis has been performed on electron micrographs of filaments of RecA on double-stranded DNA and on single-stranded DNA to visualize a difference that is consistent with one strand of the double-stranded DNA. This localization of the DNA gives additional information about the unusual structure of DNA in the complex with RecA protein.

Stay Connected to Science