Research Articles

Clathrin: a role in the intracellular retention of a Golgi membrane protein

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Science  22 Sep 1989:
Vol. 245, Issue 4924, pp. 1358-1365
DOI: 10.1126/science.2675311


Yeast mutants deficient in the clathrin heavy chain secrete a precursor form of the alpha-factor, a peptide-mating pheromone. Analysis of this defect indicates that the endoprotease Kex2p, which is responsible for initiating proteolytic maturation of the alpha-factor precursor in the Golgi apparatus, is unexpectedly present at the plasma membrane in mutant cells. This result suggest that clathrin is required for the retention of Kex2p in the Golgi apparatus.

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