Research Articles

Generation of a large combinatorial library of the immunoglobulin repertoire in phage lambda

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Science  08 Dec 1989:
Vol. 246, Issue 4935, pp. 1275-1281
DOI: 10.1126/science.2531466


A novel bacteriophage lambda vector system was used to express in Escherichia coli a combinatorial library of Fab fragments of the mouse antibody repertoire. The system allows rapid and easy identification of monoclonal Fab fragments in a form suitable for genetic manipulation. It was possible to generate, in 2 weeks, large numbers of monoclonal Fab fragments against a transition state analog hapten. The methods described may supersede present-day hybridoma technology and facilitate the production of catalytic and other antibodies.

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