Oligoclonal expansion and CD1 recognition by human intestinal intraepithelial lymphocytes

See allHide authors and affiliations

Science  20 Sep 1991:
Vol. 253, Issue 5026, pp. 1411-1415
DOI: 10.1126/science.1716785


A human intestinal intraepithelial lymphocyte (IEL) T cell line was established from jejunum to characterize the structure and function of the alpha beta T cell antigen receptors (TCRs) expressed by this population. Single-sided polymerase chain reaction (PCR) amplification cloning and quantitative PCR amplification of the TCR chains from the cell line and from fresh IELs demonstrated that IELs were oligoclonal. The IEL T cell line exhibited CD1-specific cytotoxicity and a dominant IEL T cell clone was CD1c-specific. Thus, human jejunal intraepithelial lymphocytes are oligoclonal and recognize members of the CD1 gene family.

Stay Connected to Science