GAP domains responsible for ras p21-dependent inhibition of muscarinic atrial K+ channel currents

See allHide authors and affiliations

Science  10 Jan 1992:
Vol. 255, Issue 5041, pp. 192-194
DOI: 10.1126/science.1553544


The interaction between the low molecular weight G protein ras p21 and a guanosine triphosphatase activating protein (GAP) uncouples a heterotrimeric G protein (Gk) from muscarinic receptors. Through the use of isolated atrial cell membranes and genetically engineered GAP deletion mutants, the src homology regions (SH2-SH3) at the amino terminus of GAP have been identified as the domains responsible for this effect. Deletion of the domain required to stimulate the guanosine triphosphatase activity of ras p21 relieves the requirement for ras p21 in this system. A model is presented that suggests that ras p21 induces a conformational change in GAP, which allows the SH2-SH3 regions of GAP to function.

Stay Connected to Science