Absence of p350 subunit of DNA-activated protein kinase from a radiosensitive human cell line

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Science  24 Feb 1995:
Vol. 267, Issue 5201, pp. 1183-1185
DOI: 10.1126/science.7855602


The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.

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