Yeast Checkpoint Genes in DNA Damage Processing: Implications for Repair and Arrest

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Science  01 Dec 1995:
Vol. 270, Issue 5241, pp. 1488-1491
DOI: 10.1126/science.270.5241.1488


Yeast checkpoint control genes were found to affect processing of DNA damage as well as cell cycle arrest. An assay that measures DNA damage processing in vivo showed that the checkpoint genes RAD17, RAD24, and MEC3 activated an exonuclease that degrades DNA. The degradation is probably a direct consequence of checkpoint protein function, because RAD17 encodes a putative 3′-5′ DNA exonuclease. Another checkpoint gene, RAD9, had a different role: It inhibited the degradation by RAD17, RAD24, and MEC3. A model of how processing of DNA damage may be linked to both DNA repair and cell cycle arrest is proposed.

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