Direct Visualization of A-, P-, and E-Site Transfer RNAs in the Escherichia coli Ribosome

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Science  16 Feb 1996:
Vol. 271, Issue 5251, pp. 1000-1002
DOI: 10.1126/science.271.5251.1000


Transfer RNA (tRNA) molecules play a crucial role in protein biosynthesis in all organisms. Their interactions with ribosomes mediate the translation of genetic messages into polypeptides. Three tRNAs bound to the Escherichia coli 70S ribosome were visualized directly with cryoelectron microscopy and three-dimensional reconstruction. The detailed arrangement of A- and P-site tRNAs inferred from this study allows localization of the sites for anticodon interaction and peptide bond formation on the ribosome.

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