Rearrangement-Enhancing Element Upstream of the Mouse Immunoglobulin Kappa Chain J Cluster

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Science  08 Mar 1996:
Vol. 271, Issue 5254, pp. 1416-1420
DOI: 10.1126/science.271.5254.1416


Transcriptional regulatory elements have been shown to be necessary but not sufficient for the developmental regulation of immunoglobulin gene rearrangement in mouse precursor B cells. In the chicken λ light chain locus, additional elements in the V-J intervening sequence are involved in negative and positive regulation of rearrangement. Here, mutation of the mouse homolog of a chicken element, located in the Vκ-Jκ intervening sequence upstream of the Jκ cluster, was shown to significantly decrease rearrangement. This cis-acting recombination-enhancing element affects the rearrangement process without being involved in regulating transcription.

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