Thymine-Thymine Dimer Bypass by Yeast DNA Polymerase ζ

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Science  14 Jun 1996:
Vol. 272, Issue 5268, pp. 1646-1649
DOI: 10.1126/science.272.5268.1646


The REV3 and REV7 genes of the yeast Saccharomyces cerevisiae are required for DNA damage-induced mutagenesis. The Rev3 and Rev7 proteins were shown to form a complex with DNA polymerase activity. This polymerase replicated past a thymine-thymine cis-syn cyclobutane dimer, a lesion that normally severely inhibits replication, with an efficiency of ∼10 percent. In contrast, bypass replication efficiency with yeast DNA polymerase α was no more than 1 percent. The Rev3-Rev7 complex is the sixth eukaryotic DNA polymerase to be described, and is therefore called DNA polymerase ζ.

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