Bipartite Ca2+-Binding Motif in C2 Domains of Synaptotagmin and Protein Kinase C

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Science  12 Jul 1996:
Vol. 273, Issue 5272, pp. 248-251
DOI: 10.1126/science.273.5272.248


C2 domains are found in many proteins involved in membrane traffic or signal transduction. Although C2 domains are thought to bind calcium ions, the structural basis for calcium binding is unclear. Analysis of calcium binding to C2 domains of synaptotagmin I and protein kinase C-β by nuclear magnetic resonance spectroscopy revealed a bipartite calcium-binding motif that involves the coordination of two calcium ions by five aspartate residues located on two separate loops. Sequence comparisons indicated that this may be a widely used calcium-binding motif, designated here as the C2 motif.