BTK as a Mediator of Radiation-Induced Apoptosis in DT-40 Lymphoma B Cells

See allHide authors and affiliations

Science  23 Aug 1996:
Vol. 273, Issue 5278, pp. 1096-1100
DOI: 10.1126/science.273.5278.1096


Bruton's tyrosine kinase (BTK) is a member of the SRC-related TEC family of protein tyrosine kinases (PTKs). DT-40 lymphoma B cells, rendered BTK-deficient through targeted disruption of the btk gene by homologous recombination knockout, did not undergo radiation-induced apoptosis, but cells with disrupted lyn or syk genes did. Introduction of the wild-type, or a SRC homology 2 domain or a plecstrin homology domain mutant (but not a kinase domain mutant), human btk gene into BTK-deficient cells restored the apoptotic response to radiation. Thus, BTK is the PTK responsible for triggering radiation-induced apoptosis of lymphoma B cells, and its kinase domain is indispensable for the apoptotic response.

Stay Connected to Science