Functional Analysis of the Genes of Yeast Chromosome V by Genetic Footprinting

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Science  20 Dec 1996:
Vol. 274, Issue 5295, pp. 2069-2074
DOI: 10.1126/science.274.5295.2069


Genetic footprinting was used to assess the phenotypic effects of Ty1 transposon insertions in 268 predicted genes of chromosome V of Saccharomyces cerevisiae. When seven selection protocols were used, Ty1 insertions in more than half the genes tested (157 of 268) were found to result in a detectable reduction in fitness. Results could not be obtained for fewer than 3 percent of the genes tested (7 of 268). Previously known mutant phenotypes were confirmed, and, for about 30 percent of the genes, new mutant phenotypes were identified.