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Microtubule Treadmilling in Vivo

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Science  10 Jan 1997:
Vol. 275, Issue 5297, pp. 215-218
DOI: 10.1126/science.275.5297.215

Abstract

In vivo, cytoplasmic microtubules are nucleated and anchored by their minus ends at the centrosome and are believed to turn over by a mechanism termed dynamic instability: depolymerization and repolymerization at their plus ends. In cytoplasmic fragments of fish melanophores, microtubules were shown to detach from their nucleation site and depolymerize from their minus ends. Free microtubules moved toward the periphery by treadmilling—growth at one end and shortening from the opposite end. Frequent release from nucleation sites may be a general property of centrosomes and permit a minus-end mechanism of microtubule turnover and treadmilling.

Microtubules (MTs) are fibrillar intracellular structures that play important roles in multiple cellular activities, including mitosis, transport, positioning of membrane organelles, and determination of cellular shape. MT arrays within cells are capable of rapid rearrangement that depends to a large extent on MT dynamics—the ability to exchange subunits between the soluble and polymer pools (1). Studies on MT dynamics in vitro demonstrate the existence of two principal mechanisms of subunit exchange known as treadmilling (2) and dynamic instability (3). Treadmilling involves the addition of subunits to one (plus) end of an MT and loss of subunits from the opposite (minus) end. Dynamic instability is defined by gain and loss of subunits at the same end (either plus or minus) of an MT during growth and shortening. In living cells, where the minus ends of MTs are believed to be tightly anchored at the centrosome, MTs are thought to exchange subunits by polymerization and depolymerization at their plus ends, thus using the dynamic instability mechanism (4).

We studied microtubule dynamics in cytoplasmic fragments of fish melanophores, which translocate cytoplasmic pigment granules to the center (aggregation) or to the periphery (dispersion) along a radial array of MTs (5). Remarkably, melanophore fragments retained the ability to aggregate pigment and organize a radial MT array of correct polarity orientation (with the minus ends at the center) (6) in the apparent absence of the centrosome (7). To study MT dynamics, we fluorescently tagged MTs in melanophores by microinjection of labeled tubulin, microsurgically dissected fragments from the parental cells, and induced pigment aggregation and formation of the MT aster (8). Images of labeled MTs were then sequentially acquired in the living fragments at short time intervals (3 s) for extended periods (10 min) (9).

Playback of the image sequences revealed characteristic patterns of MT behavior. At any given time, about 80% of the MTs (n = 1067) appeared to be static, with one end at the pigment aggregate and the other at the plasma membrane. The other 20% of MTs showed dynamic behavior, either primarily growing (10.6%), primarily shortening (9.3%), or moving (∼1%) (Fig. 1A). MTs showed only short length excursions at their free ends, as if dynamic instability was suppressed. MTs emerged from the pigment mass and grew toward the periphery (Fig. 1A, MT1), which indicates that the pigment aggregate had the capacity to nucleate MTs. The aggregate also seemed to stochastically release MTs, after which they shortened at their proximal ends (Fig. 1A, MT2). Thus, the two populations of MTs were interconvertible. Static MTs, after being released from the aggregate, began to shorten; growing MTs, after arriving at the surface, generally became static. In addition to growing and shortening, short MTs of constant length that moved away from the pigment aggregate were observed (Fig. 1A, MT3). This last pattern of MT behavior was so remarkable that it provoked the question of the mechanism of movement.

Fig. 1.

Microtubule behavior in melanophore fragments (14). (A) Live fluorescence images of microtubules in a fragment with pigment granules aggregated to the center. Single panel at left, MT distribution in a fragment at low magnification. Scale bar, 10 μm. Panels at right, time sequence of MTs in the same fragment at higher magnification. MT1 grows at its distal end, MT2 shortens at its proximal end, and MT3 translocates through the cytoplasm from the aggregate to the plasma membrane. MT3 was exceptional in that it failed to stop on arrival at the membrane; rather, it moved along the fragment edge, stopping at the next orthogonal surface. Time in seconds is indicated at the lower left of each panel. (B) Change of distance from the pigment aggregate of ends of the three MTs shown in (A).

Movement of MTs could be achieved either by transport (MT-dependent motors bound to a cytoplasmic matrix) or by treadmilling (polymerization at the plus end and depolymerization at the minus end). The rate of a motor-driven process need bear no relation to polymerization or depolymerization kinetics. In contrast, treadmilling is possible only if the rate of MT polymerization at the plus end equals the rate of depolymerization at the minus end. Analysis of MT length kinetics showed that, unlike the process of dynamic instability, growing MTs persisted in the growing phase (93% of the time) (Fig. 1B, MT1) and shortening MTs persisted in the shortening phase (92% of the time) (Fig. 1B, MT2). Persistent growth was always at the distal end, and persistent shortening was always at the proximal end, which is consistent with the identification of distal ends as plus and proximal ends as minus. Analysis of instantaneous growth (or shortening) rates for the MT population showed that the average rate of polymerization (v+ = 4.2 ± 4.2 μm/min) was essentially equal to the average rate of depolymerization (v = 4.4 ± 4.1 μm/min) (Fig. 2). However, the standard deviation was large, presumably because of residual dynamic instability and variation among cell fragments. Translocating MTs with both ends free provided an opportunity for closer analysis at the level of single MTs (Fig. 1B, MT3). Although MTs in different fragments varied in their absolute rate of movement, the ratio v+/v for individual MTs was essentially unity (0.99 ± 0.25) (10). Thus, the major condition of treadmilling was satisfied. Although these results strongly suggested a treadmilling mechanism for MT translocation, they did not rigorously exclude transport.

Fig. 2.

Frequency histograms of rates of MT polymerization at (A) the distal (plus) ends and depolymerization at (B) the proximal (minus) ends. Change of MT length was quantitated for 47 growing and 45 shortening MTs in 10 fragments. Rates of MT growth or shortening for each time point in the series of images were calculated with a running average of five time points.

To definitively distinguish between transport and treadmilling mechanisms, a reference mark was placed on translocating MTs by photobleaching a narrow zone with a laser microbeam. If MTs were transported, the zone of bleaching would move with the MT so that the distances between the zone and the ends of the MT would remain constant. Alternatively, if MTs treadmilled, the zone would remain stationary but the distance from the zone to the leading end would increase and the distance to the trailing end would decrease. Bleached zones placed on growing (Fig. 3, A and D) and shortening (Fig. 3, B and E) MTs did not inhibit either growth or shortening and always remained stationary. When a moving MT was irradiated with a laser microbeam (n = 6), its leading end advanced away from the photobleached zone, whereas its trailing end approached the zone, ultimately crossing it and appearing on the other side (Fig. 3, C and F), which demonstrated that MTs moved by a treadmilling mechanism.

Fig. 3.

Photobleach marking of growing, shortening, and translocating MTs (14). Fluorescently labeled MTs growing (A), shortening (B), and translocating (C) away from the pigment aggregate were crossed with the laser microbeam (vertical arrows; time in seconds is shown at the lower right of each panel). Photobleaching did not affect growth (A) and shortening (B) of MTs. Similarly to the bleached zones placed on growing (A) and shortening (B) MTs, the bleached zone placed on translocating MTs (C) remained stationary while the proximal end of the MT first approached the zone, entered it at 244 s, and appeared on its other side at 275 s. Upward-pointing arrowheads in (A) and (C) indicate distal (growing) ends of MTs; downward-pointing arrowheads in (B) and (C) indicate proximal (shortening) ends. (D through F). Change of distance from reference points of ends of MTs shown in (A) through (C). The length and position of the MTs in (F) are shown by the thin vertical lines. The positions and duration of the bleached zones are shown as open horizontal bars. (G and H) MT life cycle. (G) Primary pattern of MT turnover. MTs are nucleated at the pigment aggregate (black disk) and grow at their plus (distal) ends toward the surface. Growth is terminated when their distal ends reach the surface; MTs then appear stationary. Eventually, minus (proximal) ends of stationary MTs are released from the aggregate and MTs depolymerize. (H) Secondary pattern of MT turnover. Same as for primary pattern, but MT minus ends release from the pigment aggregate before their plus ends reach the surface. Because the rates of polymerization and depolymerization at the opposite ends of MTs are balanced, they treadmill toward the surface without substantial change in length. The processes involved in both patterns are the same; the only difference is whether release at the minus end follows or precedes arrival of the plus end at the surface. A residence time for the MT minus end at the pigment aggregate may be calculated from experimental parameters (11). For a fragment with a radius of 20 μm, polymerization (or depolymerization) velocities of ∼4 μm/min mean that MTs would grow (or shorten) for 5 min before arriving at the surface (or disappearing). Treadmilling MTs would appear if they were released from the aggregate within 5 min of their nucleation.

Our results permit a characterization of the MT life cycle in melanophore fragments (Fig. 3, G and H). MTs are nucleated at the pigment aggregate and grow radially outward, and their plus ends become stabilized at the plasma membrane. At some point, the minus ends are released from the aggregate and shorten toward the membrane, and the MT disappears (Fig. 3G). If MTs are released from the aggregate before their plus ends reach the plasma membrane, they treadmill to the periphery (Fig. 3H). The model predicts that in the steady state, the number of growing and shortening MTs should be the same, which was in fact observed. The two behavior patterns can be related to each other by postulating a “residence time” for an MT minus end to remain at the aggregate before it is released. Using a simple model (11) and experimental parameters, we calculated the average residence time to be 43 ± 16 min.

Why do melanophore fragments display an opposite-end pattern of MT turnover instead of dynamic instability? In cytoplasmic fragments, the pigment aggregate rather than the centrosome serves as a site for MT nucleation. Presumably, MTs are more weakly attached to pigment granules than to the centrosome and thus have a higher probability of being released. Frequent release, followed by depolymerization of MTs at their minus ends, would have the consequence of increasing the steady-state concentration of tubulin monomers. We observed depolymerization rates of 4 μm/min, which is more than two orders of magnitude faster than the treadmilling reported in vitro (2). At such rates, the predicted increase in tubulin concentration might be sufficient to significantly reduce the frequency of MT catastrophe, suppressing dynamic instability. Thus, decrease in the residence time at the nucleation site may provide a mechanism for switching the pattern of MT turnover.

Treadmilling of free MTs does not preclude similar behavior by attached MTs. Tubulin flux or treadmilling has been described for MTs of the mitotic spindle (12). These differ from free MTs in that their ends are anchored at the kinetochore and spindle pole, and their apparent treadmilling has been interpreted to be the result of coupled motor activity (12). In interphase cells, MTs are generally considered to be anchored to the centrosome. However, the literature contains a number of reports suggesting that MTs can be released (13). Our discovery of treadmilling of free MTs in vivo focuses increased attention on the MT minus end and underscores the importance of understanding how it interacts with the centrosome.

REFERENCES AND NOTES

  1. 1.
  2. 2.
  3. 3.
  4. 4.
  5. 5.
  6. 6.
  7. 7.
  8. 8.
  9. 9.
  10. 10.
  11. 11.
  12. 12.
  13. 13.
  14. 14.
  15. 15.
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