Inhibition of Invasion of Epithelial Cells by Tiam1-Rac Signaling

See allHide authors and affiliations

Science  21 Nov 1997:
Vol. 278, Issue 5342, pp. 1464-1466
DOI: 10.1126/science.278.5342.1464

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution


Tiam1 encodes an exchange factor for the Rho-like guanosine triphosphatase Rac. Both Tiam1 and activated RacV12 promote invasiveness of T lymphoma cells. In epithelial Madin–Darby canine kidney (MDCK) cells, Tiam1 localized to adherens junctions. Ectopic expression of Tiam1 or RacV12 inhibited hepatocyte growth factor–induced scattering by increasing E-cadherin–mediated cell-cell adhesion accompanied by actin polymerization at cell-cell contacts. In Ras-transformed MDCK cells, expression of Tiam1 or RacV12 restored E-cadherin–mediated adhesion, resulting in phenotypic reversion and loss of invasiveness. These data suggest an invasion-suppressor role for Tiam1 and Rac in epithelial cells.

  • * Present address: Central Laboratory of the Red Cross Blood Transfusion Services, Plesmanlaan 125, 1066 CX Amsterdam, Netherlands.

View Full Text

Stay Connected to Science