Exon Shuffling by L1 Retrotransposition

See allHide authors and affiliations

Science  05 Mar 1999:
Vol. 283, Issue 5407, pp. 1530-1534
DOI: 10.1126/science.283.5407.1530

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution


Long interspersed nuclear elements (LINE-1s or L1s) are the most abundant retrotransposons in the human genome, and they serve as major sources of reverse transcriptase activity. Engineered L1s retrotranspose at high frequency in cultured human cells. Here it is shown that L1s insert into transcribed genes and retrotranspose sequences derived from their 3′ flanks to new genomic locations. Thus, retrotransposition-competent L1s provide a vehicle to mobilize non-L1 sequences, such as exons or promoters, into existing genes and may represent a general mechanism for the evolution of new genes.

  • * Present address, Departments of Human Genetics and Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, 48109–0650 USA.

  • To whom correspondence should be addressed. E-mail: moranj{at} or kazazian{at}

View Full Text

Stay Connected to Science