Three-Dimensional Structures of the TAFII-Containing Complexes TFIID and TFTC

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Science  10 Dec 1999:
Vol. 286, Issue 5447, pp. 2151-2153
DOI: 10.1126/science.286.5447.2151

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TBP (TATA-binding protein)–associated factors (TAFIIs) are components of large multiprotein complexes such as TFIID, TFTC, STAGA, PCAF/GCN5, and SAGA, which play a key role in the regulation of gene expression by RNA polymerase II. The structures of TFIID and TFTC have been determined at 3.5-nanometer resolution by electron microscopy and digital image analysis of single particles. Human TFIID resembles a macromolecular clamp that contains four globular domains organized around a solvent-accessible groove of a size suitable to bind DNA. TFTC is larger and contains five domains, four of which are similar to TFIID.

  • * These authors contributed equally to this work.

  • To whom correspondence should be addressed. E-mail: pat{at}

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