Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression

See allHide authors and affiliations

Science  02 Jun 2000:
Vol. 288, Issue 5471, pp. 1643-1647
DOI: 10.1126/science.288.5471.1643

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution

This article has a correction. Please see:


Little is known about the DNA helicases required for the elongation phase of eukaryotic chromosome replication. Minichromosome maintenance (MCM) protein complexes have DNA helicase activity but have only been functionally implicated in initiating DNA replication. Using an improved method for constructing conditional degron mutants, we show that depletion of MCMs after initiation irreversibly blocks the progression of replication forks in Saccharomyces cerevisiae. Like the Escherichia coli dnaB and SV40 T antigen helicases, therefore, the MCM complex is loaded at origins before initiation and is essential for elongation. Restricting MCM loading to the G1 phase ensures that initiation and elongation occur just once per cell cycle.

  • * To whom correspondence should be addressed. E-mail: J.Diffley{at}

View Full Text

Stay Connected to Science