Induction of Mating in Candida albicans by Construction of MTLa and MTLα Strains

See allHide authors and affiliations

Science  14 Jul 2000:
Vol. 289, Issue 5477, pp. 310-313
DOI: 10.1126/science.289.5477.310

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution


Although the diploid fungus Candida albicans, a human pathogen, has been thought to have no sexual cycle, it normally possesses mating-type–like orthologs (MTL) of both of theSaccharomyces cerevisiae mating-type genes (MAT) a and α. When strains containing only MTL a or MTLα were constructed by the loss of one homolog of chromosome 5, the site of theMTL loci, MTL a and MTLα strains mated, but like mating types did not. Evidence for mating included formation of stable prototrophs from strains with complementing auxotrophic markers; these contained both MTLalleles and molecular markers from both parents and were tetraploid in DNA content and mononucleate.

  • * To whom correspondence should be addressed. E-mail: ptm{at}

View Full Text

Stay Connected to Science