Interactions of the COP9 Signalosome with the E3 Ubiquitin Ligase SCFTIR1 in Mediating Auxin Response

Claus Schwechheimer; Giovanna Serino; Judy Callis; William L. Crosby; Svetlana Lyapina; Raymond J. Deshaies; William M. Gray; Mark Estelle; Xing-Wang Deng

doi:10.1126/science.1059776

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Abstract

The COP9 signalosome is an evolutionary conserved multiprotein complex of unknown function that acts as a negative regulator of photomorphogenic seedling development in Arabidopsis. Here, we show that plants with reduced COP9 signalosome levels had decreased auxin response similar to loss-of-function mutants of the E3 ubiquitin ligase SCF^TIR1. Furthermore, we found that the COP9 signalosome and SCF^TIR1 interacted in vivo and that the COP9 signalosome was required for efficient degradation of PSIAA6, a candidate substrate of SCF^TIR1. Thus, the COP9 signalosome may play an important role in mediating E3 ubiquitin ligase–mediated responses.

↵* These authors contributed equally to this work.
↵† To whom correspondence should be addressed. E-mail: xingwang.deng{at}yale.edu

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Interactions of the COP9 Signalosome with the E3 Ubiquitin Ligase SCF^TIR1 in Mediating Auxin Response

Abstract

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