Correlation Between Histone Lysine Methylation and Developmental Changes at the Chicken β-Globin Locus

See allHide authors and affiliations

Science  28 Sep 2001:
Vol. 293, Issue 5539, pp. 2453-2455
DOI: 10.1126/science.1064413

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution


Methylation of histones at specific residues plays an important role in transcriptional regulation. Chromatin immunoprecipitation of dimethylated lysine 9 on histone H3 across 53 kilobases of the chicken β-globin locus during erythropoiesis shows an almost complete anticorrelation between regions of elevated lysine 9 methylation and acetylation. Lysine 9 is methylated most over constitutive condensed chromatin and developmentally inactive globin genes. In contrast, lysine 4 methylation of histone H3 correlates with H3 acetylation. These results lead us to propose a mechanism by which the insulator in the β-globin locus can protect the globin genes from being silenced by adjacent condensed chromatin.

  • * To whom correspondence should be addressed. E-mail: gxf{at}

View Full Text

Stay Connected to Science