Production of α-1,3-Galactosyltransferase Knockout Pigs by Nuclear Transfer Cloning

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Science  08 Feb 2002:
Vol. 295, Issue 5557, pp. 1089-1092
DOI: 10.1126/science.1068228

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The presence of galactose α-1,3-galactose residues on the surface of pig cells is a major obstacle to successful xenotransplantation. Here, we report the production of four live pigs in which one allele of the α-1,3-galactosyltransferase locus has been knocked out. These pigs were produced by nuclear transfer technology; clonal fetal fibroblast cell lines were used as nuclear donors for embryos reconstructed with enucleated pig oocytes.

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