Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification Pathway

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Science  15 Feb 2002:
Vol. 295, Issue 5558, pp. 1294-1298
DOI: 10.1126/science.1067765

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The Nedd8 ubiquitin-like protein modification pathway regulates cell-cycle progression. Our analysis of Nedd8 requirements duringCaenorhabditis elegans embryogenesis indicates that the cytoskeleton is another target. Nedd8 conjugation negatively regulated contractility of the microfilament-rich cell cortex during pronuclear migration and again during cytokinesis. The Nedd8 pathway also was required after meiosis to negatively regulate katanin, a microtubule-severing complex, permitting the assembly of a large mitotic spindle. We propose that Nedd8-modified cullin, as part of an E3 ubiquitin ligase complex, targets katanin for degradation during the transition from meiosis to mitosis.

  • * Present address: Department of Zoology, Ohio Wesleyan University, Delaware, OH 43015, USA.

  • To whom correspondence should be addressed. E-mail: bbowerman{at}

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