Dynamics of Thymocyte-Stromal Cell Interactions Visualized by Two-Photon Microscopy

See allHide authors and affiliations

Science  07 Jun 2002:
Vol. 296, Issue 5574, pp. 1876-1880
DOI: 10.1126/science.1070945

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution


Thymocytes are selected to mature according to their ability to interact with self major histocompatibility complex (MHC)–peptide complexes displayed on the thymic stroma. Using two-photon microscopy, we performed real-time analysis of the cellular contacts made by developing thymocytes undergoing positive selection in a three-dimensional thymic organ culture. A large fraction of thymocytes within these cultures were highly motile. MHC recognition was found to increase the duration of thymocyte–stromal cell interactions and occurred as both long-lived cellular associations displaying stable cell-cell contacts and as shorter, highly dynamic contacts. Our results identify the diversity and dynamics of thymocyte interactions during positive selection.

  • * This author made independent, essential contributions to this work.

  • To whom correspondence should be addressed. E-mail: erobey{at}

View Full Text

Stay Connected to Science