Molecular Biology

Human Mediation

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Science  14 Jun 2002:
Vol. 296, Issue 5575, pp. 1933
DOI: 10.1126/science.296.5575.1933c

Several classes of protein complexes are needed to turn on gene expression, including general transcription factors, activators, and coactivators. Mediator is a coactivator complex in yeast that does not bind directly to the promoter region of the DNA, but instead acts as a bridge between activators and the COOH-terminal domain (CTD) of RNA polymerase II (the core of the transcription machine). The human co-activator required for Sp1 activation (CRSP) is known to interact with activators, and Näär et al. show that it binds to the CTD of human RNA polymerase II. Thus, although human CRSP and yeast Mediator are dissimilar in polypeptide composition and structure, they appear to function in similar ways by helping to recruit the RNA polymerase machine to the promoter. — BAP

Genes Dev. 16, 1339 (2002).

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