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Hybridization and the Evolution of Reef Coral Diversity

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Science  14 Jun 2002:
Vol. 296, Issue 5575, pp. 2023-2025
DOI: 10.1126/science.1069524

Abstract

Hundreds of coral species coexist sympatrically on reefs, reproducing in mass-spawning events where hybridization appears common. In the Caribbean, DNA sequence data from all three sympatricAcropora corals show that mass spawning does not erode species barriers. Species A. cervicornis and A. palmata are distinct at two nuclear loci or share ancestral alleles. Morphotypes historically given the name Acropora prolifera are entirely F1 hybrids of these two species, showing morphologies that depend on which species provides the egg for hybridization. Although selection limits the evolutionary potential of hybrids, F1 individuals can reproduce asexually and form long-lived, potentially immortal hybrids with unique morphologies.

Diverse reef-building coral assemblages have served as the foundation for complex reef ecosystems with exceptional biodiversity and productivity. Yet, the evolutionary genesis of coral diversity remains mired in a paradox. As many as 105 coral species from 36 genera and 11 families reproduce in yearly, synchronous mass-spawning events (1), thereby providing overwhelming opportunities for hybridization among congenerics (2). Laboratory crosses from a number of mass-spawning genera demonstrate that viable hybrids occur among congenerics (2, 3). Interspecific hybridization should blur coral species boundaries and stifle species diversification, yet many mass-spawning coral groups have rapidly diversified. The juxtaposition of high hybridization potential and high species diversity in mass-spawning corals has confused the picture of coral evolution and cast such doubt on the cohesiveness of coral species boundaries (4) that some species-rich genera have been considered hybrid swarms (3). Acropora, the world's most speciose coral group (5), exemplify this view (2–4). Most of the 115 species ofAcropora arose over the past 5 million years (My) (6, 7), and many are capable of hybridizing with sympatric congenerics in laboratory crosses (2, 8). One prominent hypothesis proposes that interspecific hybridization promotes reticulate evolution and morphological diversification in the absence of genetically distinct species (3), even though a genetic mechanism for this hypothesis is lacking. Polyphyletic sequence data for corals continue to be taken as direct evidence of reticulate evolution (8–11) without due consideration to alternatives such as incomplete lineage sorting.

To examine the potential role of hybridization in coral speciation, we analyzed DNA sequence variation at three loci in the three sympatric species of Caribbean Acropora (Fig. 1). Acropora cervicornis and A. palmata are sister species with fossil records dating back at least 3 to 3.6 My (12, 13). Both have distinct morphologies and habitat preferences. The arborescent “staghorn” coral A. cervicornis occurs throughout forereef and backreef habitats, whereas the robust “elkhorn” coral A. palmataoccurs primarily in high–wave energy reef-crest habitats (14, 15). Both species spawn synchronously over a few nights each summer (16) and can potentially hybridize. The third species, Acropora prolifera, occurs Caribbean-wide, where it varies from being locally rare to occurring in large patches (7, 14, 15). It is morphologically intermediate between A. cervicornis andA. palmata, causing many to consider it a species of hybrid origin (7, 15). Pax-C intron data showing high heterozygosity support this possibility (10). Morphological variation in A. prolifera is high and yet surprisingly discrete. In Puerto Rico, for example, there are two discrete A. prolifera morphs—a thin, highly branched form we term the “bushy” morph (Fig. 1C), and a thicker form with palmate, flattened branches we call the “palmate” morph (Fig. 1D).

Figure 1

The Caribbean Acropora species: (A) A. cervicornis and (B) A. palmata, and (C) the bushy and (D) palmate F1 hybrid A. prolifera morphs from Puerto Rico.

We obtained sequence data for the Caribbean Acroporaspecies at introns of the nuclear minicollagen and calmodulin genes, and at the mitochondrial putative control region (17). The nuclear data indicate that the speciesA. cervicornis and A. palmata are genetically distinct and that the morphologically intermediate species A. prolifera is actually a first-generation (F1) hybrid.Acropora cervicornis and A. palmata were reciprocally monophyletic at minicollagen (Fig. 2A). All of the A. prolifera(n = 22) were heterozygous at minicollagen, containing one allele from each of the two species' clades. The calmodulin data for A. cervicornis and A. palmata formed three distinct alleles: A, B, and B′ (Fig. 2B). Allele A was exclusive toA. cervicornis. B alleles were exclusive to A. palmata, but the variant B′ was shared between species, making it either a shared ancestral allele or an introgressed allele from recent or historical hybridization. As with minicollagen, all of the A. prolifera (n = 28) were heterozygous at calmodulin (A/B = 26; B/B′ = 2). The complete heterozygosity of A. prolifera at these two nuclear loci strongly suggests that every individual sampled was a F1 hybrid.

Figure 2

Maximum likelihood (ML) trees for (A) minicollagen, (B) calmodulin, and (C) mitochondrial putative control region. Likelihood searches were conducted in PAUP* 4.0b8 (31) with estimated model parameters and 25 random-addition heuristic searches with tree-bisection-reconnection branch swapping. Models of sequence evolution were evaluated on distance-based topologies with hierarchical likelihood ratio tests (32) in MODELTEST 3.06 (33). Major allele/haplotype clades are labeled. Tick marks along major branches indicate substitutions. Sample sizes (alleles or haplotypes) are labeled in parentheses (n). Site abbreviations: Yucatan (Y); Panama (Pa); Jamaica (Ja); Puerto Rico (PR); St. Croix (SC). Bootstrap values (>50%) from 300 replicates are labeled on relevant nodes. The Pacific congenerAcropora nasuta was used as the outgroup. Sequences are available in GenBank (accession numbers AF507116 to AF507373). (A) Minicollagen ML tree constructed with a K80 model (ln score = 654.81). (B) Calmodulin ML tree constructed with a HKY model (1 of 4 trees; ln score = 592.86). (C) Mitochondrial putative control region ML tree constructed with a F81 + Γ model (ln score = 2014.96). Palmate A. prolifera hybrids are shown in blue; bushy hybrids are in red.

Mitochondrial data show that the 45 unique haplotypes form a polytomy with three clades (Fig. 2C), labeled as haplotypes A, B, and C. The A and C haplotypes contained only A. cervicornis and hybridA. prolifera. The B haplotypes contained all three taxa:A. palmata, A. cervicornis, and hybrid A. prolifera. All three haplotypes were found in A. prolifera, indicating that hybrid crosses occur in both directions. Hybrids receive maternally inherited mitochondrial DNAs from either A. palmata (B haplotype) or A. cervicornis (A haplotype) “mothers.”

Although hybrid crosses occur in either direction, mitochondrial DNA (mtDNA) introgression appears unidirectional because A. cervicornis colonies possess all three haplotype clades, butA. palmata colonies do not. The data indicate that “palmata” (B) haplotypes are passed to A. cervicornis through backcrossing of A. cervicornis with hybrid A. prolifera. Introgressed B haplotypes in A. cervicornis were common (∼20%) and sampled at every site. The presence of multiple B variants in A. cervicornis indicates the mtDNA introgression has occurred more than once. Because nuclear loci should sort more slowly than maternally inherited mtDNAs (18, 19), polyphyletic patterns in the mitochondrial data but not the minicollagen data are consistent with recent introgression rather than incomplete lineage sorting.

In Puerto Rico, we sampled two distinct morphs of A. prolifera, i.e., the bushy and palmate morphs (Fig. 1, C and D). Although all individuals, irrespective of morphology, are F1 hybrids, they differ in which species donated its egg and mitochondrion to the hybridization event. All bushy hybrids had apalmata maternal and mitochondrial background, whereas all of the palmate hybrids had a cervicornis background. This suggests that maternal and/or cytoplasmic effects account for the marked differences in these two hybrid morphotypes. Thus, coral morphology appears sensitive to not only nuclear genetic effects, but also to nuclear-cytoplasmic interactions within a hybrid nuclear genome.

Differential introgression of loci characterizes many terrestrial hybridization systems (20); however, a rarely explored alternative is that the pattern is due to ancestral polymorphism. We applied a two-population Bayesian coalescent model (21) to our data and the published Pax-C data (10) to estimate the rate of introgression [as migration (M) in units 2 × the product of effective population size (Ne ) and migration (m)] and test null hypotheses of no introgression (M = 0) using likelihood ratio tests (LRTs) (22). Results [Table 1 and supplemental material (23)] indicate that the mitochondrial data are consistent with low levels of introgression (M = 0.20), roughly equivalent to one haplotype crossing the species boundary every 5N f (i.e., mtDNA effective population size) generations. For the nuclear loci, the Pax-C data were also consistent with low levels of introgression (M = 0.30), whereas the minicollagen and calmodulin data were both consistent with no introgression, suggesting that the shared B′ allele at calmodulin is a retained ancestral allele. Such differential cytoplasmic and nuclear introgression is consistent with selection against hybrid genotypes that is thought to result from selection against nuclear genes in foreign genetic backgrounds (24), and/or the breakup of coadapted gene complexes in backcrossed individuals (25).

Table 1

Estimated genetic introgression. Results of the Bayesian coalescent modeling for each gene showing the estimated rates of introgression (M in 2Nem units) and the results of the likelihood ratio tests (LRTs). NS, not significant;*P = 0.05; **P = 0.01.

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The existence of hybrid A. prolifera shows that complete barriers to hybridization have not evolved between A. palmata and A. cervicornis. However, the observation that A. prolifera hybrid populations are composed almost entirely of F1 individuals suggests that the reproductive potential of hybrid A. prolifera is severely limited or that hybrid breakdown occurs in later generations. Some hybrid A. prolifera are reproductive, produce viable gametes, and are interfertile with A. cervicornis. Yet, the limited introgression suggests that they are essentially sterile “mules,” which have little genetic impact on either parent species. Strict F1 hybrids are often ecologically rare in natural hybridization systems (26). Where F1hybrids dominate, selection manifest as hybrid infertility or hybrid breakdown has been inferred, as here (27). Such F1 hybrids should be common only when hybridization is frequent or F1 offspring are long-lived. Like many corals (28), hybrid A. prolifera can propagate clonally by fragmentation (29), allowing for long-lived, potentially immortal hybrid genotypes. These “immortal mules” may accumulate over time, providing the opportunity for rare backcrosses, and for the ecological persistence of a diverse suite of Acroporamorphotypes that is greater than the number of species on reefs.

The Caribbean Acropora show that reef-building corals diversify not only through conventional species formation, but also through the unprecedented formation of long-lived coral hybrid morphotypes. In effect, hybridization, through the formation of asexual coral hybrid lines, generates new morphologies and potentially new ecotypes without speciation. Similar clonal niche partitioning is known for rare parthenogenetic taxa (30), but has never been postulated for an ecosystem-defining group like reef-building corals. Although it remains to be seen how pervasive coral hybrid “mules” are, the variety of intermediate morphologies in corals, especially in regional endemics and putative subspecies (5), suggests that morphologically unique hybrids may be common. Because of the potential for natural hybridization in mass-spawning corals, the coral reticulate evolution hypothesis suggested that genetic exchange between “species” generates discrete coral morphologies (3) without genetic isolation. Instead, we suggest that reef-building coral diversity is enhanced by hybridization through the production of long-lived asexual hybrid morphotypes, which have little evolutionary potential.

  • * To whom correspondence should be addressed. E-mail: svollmer{at}oeb.harvard.edu

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