RNA Polymerase II Transcription in Murine Cells Lacking the TATA Binding Protein

See allHide authors and affiliations

Science  01 Nov 2002:
Vol. 298, Issue 5595, pp. 1036-1039
DOI: 10.1126/science.1076327

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution


Inactivation of the murine TATA binding protein (TBP) gene by homologous recombination leads to growth arrest and apoptosis at the embryonic blastocyst stage. However, after loss of TBP, RNA polymerase II (pol II) remains in a transcriptionally active phosphorylation state, and in situ run-on experiments showed high levels of pol II transcription comparable to those of wild-type cells. In contrast, pol I and pol III transcription was arrested. Our results show a differential dependency of the RNA polymerases on TBP and provide evidence for TBP-independent pol II transcriptional mechanisms that allow reinitiation and maintenance of gene transcription in vivo.

  • * To whom correspondence should be addressed. E-mail: irwin{at}

View Full Text

Stay Connected to Science