Fluorometric Detection of Enzyme Activity with Synthetic Supramolecular Pores

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Science  22 Nov 2002:
Vol. 298, Issue 5598, pp. 1600-1602
DOI: 10.1126/science.1077353

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The reversible blockage of synthetic pores formed by rigid-rod β barrels, either by substrates or products, was used to sense a variety of enzymatic reactions in high-throughput format with “naked-eye” fluorescent detection. Improvement of sensor sensitivity beyond three orders of magnitude by straightforward internal mutations underscores the functional plasticity of rigid-rod β barrels. Such detectors of enzyme activity with the aforementioned characteristics are needed in areas as diverse as proteomics and environmentally benign organic synthesis.

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