Caveolae Conducting Calcium

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Science  29 Nov 2002:
Vol. 298, Issue 5599, pp. 1683
DOI: 10.1126/science.298.5599.1683c

Caveolae are specialized membrane domains that are thought to function at the plasma membrane in endocytosis and have been found to contain relatively high concentrations of signaling molecules.

Isshiki et al. now present evidence that caveolae serve as preferential sites of Ca2+ influx across the plasma membrane when intracellular stores of Ca2+ are depleted. The calcium sensor yellow chameleon 3.1 was fused to the COOH-terminal end of caveolin-1 to target the sensor to caveolae. Ca2+ signals at the caveolae were compared to those from unmodified yellow chameleon in the cytoplasm or from another fusion protein that targeted the sensor to the plasma membrane. When Ca2+ was depleted from the endoplasmic reticulum of fetal bovine endothelial cells, Ca2+ entry into the cells occurred preferentially at caveolae, apparently though store-operated channels of the TRP family. — LBR

J. Biol. Chem.277, 43389 (2002).

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