In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles

See allHide authors and affiliations

Science  29 Nov 2002:
Vol. 298, Issue 5599, pp. 1759-1762
DOI: 10.1126/science.1077194

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution


Fluorescent semiconductor nanocrystals (quantum dots) have the potential to revolutionize biological imaging, but their use has been limited by difficulties in obtaining nanocrystals that are biocompatible. To address this problem, we encapsulated individual nanocrystals in phospholipid block–copolymer micelles and demonstrated both in vitro and in vivo imaging. When conjugated to DNA, the nanocrystal-micelles acted as in vitro fluorescent probes to hybridize to specific complementary sequences. Moreover, when injected into Xenopus embryos, the nanocrystal-micelles were stable, nontoxic (<5 × 109 nanocrystals per cell), cell autonomous, and slow to photobleach. Nanocrystal fluorescence could be followed to the tadpole stage, allowing lineage-tracing experiments in embryogenesis.

  • * To whom correspondence should be addressed. E-mail: benoit.dubertret{at}, dnorris{at}

  • Present address: UPRA0005, CNRS and ESPCI, Laboratoire d'Optique Physique, 10 rue Vauquelin, 75005 Paris, France.

View Full Text

Stay Connected to Science