Role of EDEM in the Release of Misfolded Glycoproteins from the Calnexin Cycle

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Science  28 Feb 2003:
Vol. 299, Issue 5611, pp. 1397-1400
DOI: 10.1126/science.1079474

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The mechanisms that determine how folding attempts are interrupted to target folding-incompetent proteins for endoplasmic reticulum–associated degradation (ERAD) are poorly defined. Here the α-mannosidase I–like protein EDEM was shown to extract misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. EDEM overexpression resulted in faster release of folding-incompetent proteins from the calnexin cycle and earlier onset of degradation, whereas EDEM down-regulation prolonged folding attempts and delayed ERAD. Up-regulation of EDEM during ER stress may promote cell recovery by clearing the calnexin cycle and by accelerating ERAD of terminally misfolded polypeptides.

  • * To whom correspondence should be addressed. E-mail: Maurizio.molinari{at}

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