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Transmission of Equine Influenza Virus to Dogs

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Science  21 Oct 2005:
Vol. 310, Issue 5747, pp. 482-485
DOI: 10.1126/science.1117950

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  1. Fig. 1.

    Phylogenetic relationships among the hemagglutinin genes. (A) Tree of HA genes from representative canine, human, avian, swine, and equine isolates, using A/budgerigar/Hokkaido/1/77 (H4) as the outgroup (indicated by arrowhead and H4). (B) Tree of the canine influenza virus HA genes with contemporary and older equine HA genes, using A/duck/Ukraine/63 (H3) as the outgroup. Phylogenetic trees were inferred from nucleotide sequences by the neighbor joining method, and bootstrap analysis values ≥90% are shown. The bar denotes the number of nucleotide changes per unit length of the horizontal tree branches.

  2. Fig. 2.

    Immunohistochemical detection of influenza H3 antigen in the lungs. Lung tissue sections were probed with a mouse monoclonal antibody to H3 hemagglutinin and binding was detected by immunoperoxidase reaction (brown precipitate). (A) Bronchial epithelium from a greyhound with disease. Viral H3 antigen was detected in bronchial epithelial cell cytoplasm and in macrophages in airway lumens and in alveolar spaces. (B) Bronchial epithelium from a beagle dog 5 days after inoculation with A/canine/Florida/43/04 (H3N8). Viral H3 antigen was detected in bronchial epithelial cell cytoplasm. Scale bar, 66 μm.

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