PerspectiveBiochemistry

RNA Polymerase, a Scrunching Machine

See allHide authors and affiliations

Science  17 Nov 2006:
Vol. 314, Issue 5802, pp. 1097-1098
DOI: 10.1126/science.1135746

You are currently viewing the figures only.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution

  1. Ready to scrunch.

    The model shows an open promoter complex of bacterial RNA polymerase (RNAP) poised to begin RNA synthesis, with arrows designating motions of DNA segments that occur as the first nucleotides are combined to form an RNA chain. Downstream DNA (on the right) rotates inward and separates in the active site channel of the polymerase. Template (orange) and nontemplate (pink) DNA strands follow the indicated paths, moving as the RNA chain (not shown) is polymerized on the template DNA strand. Sites of extrusion of single-stranded DNA are shown by outlined arrows at the top. σ70 (blue); RNAP core chains (gray); a fluorescent tag on σ70 region 2 used for FRET (green); a fluorescent tag on downstream DNA (initial position in yellow; scrunched position in red).

    CREDIT: A. KAPANIDIS/UNIVERSITY OF OXFORD; S. WEISS/UNIVERSITY OF CALIFORNIA, LOS ANGELES; AND R. H. EBRIGHT/RUTGERS UNIVERSITY

Stay Connected to Science