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Dissecting the Functions of the Mammalian Clock Protein BMAL1 by Tissue-Specific Rescue in Mice

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Science  24 Nov 2006:
Vol. 314, Issue 5803, pp. 1304-1308
DOI: 10.1126/science.1132430

Abstract

The basic helix-loop-helix (bHLH)–Per-Arnt-Sim (PAS) domain transcription factor BMAL1 is an essential component of the mammalian circadian pacemaker. Bmal1–/– mice lose circadian rhythmicity but also display tendon calcification and decreased activity, body weight, and longevity. To investigate whether these diverse functions of BMAL1 are tissue-specific, we produced transgenic mice that constitutively express Bmal1 in brain or muscle and examined the effects of rescued gene expression in Bmal1–/– mice. Circadian rhythms of wheel-running activity were restored in brain-rescued Bmal1–/– mice in a conditional manner; however, activity levels and body weight were lower than those of wild-type mice. In contrast, muscle-rescued Bmal1–/– mice exhibited normal activity levels and body weight yet remained behaviorally arrhythmic. Thus, Bmal1 has distinct tissue-specific functions that regulate integrative physiology.

Circadian rhythms control many aspects of mammalian physiology and behavior. The suprachiasmatic nuclei (SCN) act as pacemakers required for the generation of circadian behavioral rhythms as well as synchronizers of autonomous peripheral tissue clocks (1). Molecular circadian regulation engages a transcription-translation feedback loop comprising the activating proteins CLOCK and BMAL1, which induce expression of the negative feedback elements Per and Cry (1). BMAL1 (also known as MOP3) was originally characterized by its high expression in brain and muscle (2, 3) and was identified as a heterodimeric binding partner of CLOCK (4, 5). Bmal1–/– mice not only lose behavioral circadian rhythmicity but also exhibit a variety of other phenotypes including decreased activity levels and body weight, progressive joint disease, and shortened life span (612). Therefore, in addition to circadian rhythm regulation, BMAL1 appears to play a role in a variety of functions that are potentially dependent on the tissue type in which it is expressed. To determine whether BMAL1 has unique tissue-specific functions, we generated transgenic mice that express Bmal1 ubiquitously or in distinct tissue types. We then crossed these lines onto a Bmal1 null background and determined which phenotypes could be rescued by exogenous, tissue-specific Bmal1 expression.

We first examined the effects of rescuing Bmal1 ubiquitously by using a transgenic mouse line produced with Bmal1-containing bacterial artificial chromosome (BAC) clones (Fig. 1A) (13). Because the BAC clones contain the genomic coding and promoter sequence of Bmal1, expression of the transgene should occur in all tissues that normally express Bmal1. We measured increased Bmal1 expression in the SCN of BAC transgenic mice at normal peak and trough times of Bmal1 mRNA [ZT (zeitgeber time) 18 and 6, respectively], and also observed increased Bmal1 expression during peak times in the liver (ZT 18 to ZT 2) (fig. S1) (13). BAC transgenic mice were then sequentially crossed with Bmal1+/– mice to produce BAC-rescued Bmal1–/– mice (13). Circadian rhythms of locomotor activity were then analyzed in a 12 hour:12 hour light:dark (LD) cycle followed by constant darkness (DD) conditions (Fig. 1C) (13).

Fig. 1.

Bmal1-containing BAC transgenes rescue Bmal1–/– phenotypes. (A) Bmal1 BAC clones were used to create transgenic (Tg) mice, which were consecutively crossed with Bmal1+/– mice to create BAC-rescued Bmal1–/– mice. (B) Bmal1 mRNA levels in SCN were examined by in situ hybridization in wild-type (WT) and Bmal1 BAC Tg mice, killed at ZT 6 and ZT 18 [shown are means ± SEM; significant effect of genotype, generalized linear model analysis of variance (GLM ANOVA)]. (C) Representative wheel-running activity records from WT, Bmal1 BAC Tg, Bmal1–/– (Bmal1 KO), or BAC-rescued (Bmal1 BAC Tg; Bmal1 KO) mice. Mice were housed in LD and then released into DD for 3 weeks. (D) Bar graphs of means ± SEM show that BAC-rescued mice (n = 6) exhibit free-running period, amplitude of circadian rhythm, and activity levels that are not significantly different from those of WT. Amplitude is graphed as the peak amplitude of the proportion of the total variance in the time series in the circadian (∼24 hours) range (***P < 0.001, one-way ANOVA; *significant effect of genotype, GLM ANOVA).

Whereas Bmal1–/– mice exhibited no circadian rhythm of activity in DD and showed reduced activity levels, BAC-rescued Bmal1–/– mice displayed normal circadian rhythm characteristics (free-running period and amplitude of circadian rhythm) and activity levels in LD and DD that were similar to those of wild-type mice (Fig. 1D). In addition, 100% of BAC-rescued Bmal1–/– mice survived until the end of experimental analysis (≥10 months old) compared to 29% of Bmal1–/– mice. Therefore, long-term survival was restored in the BAC-rescued Bmal1–/– mice, and no gross abnormalities such as low body weight or joint calcification were observed in the BAC-rescued mice. Thus, Bmal1 BAC transgenes completely rescued the phenotypes observed in Bmal1–/– mice.

We next determined whether expression of Bmal1 in brain tissue could restore behavioral rhythms in Bmal1–/– mice as well as alleviate other phenotypes. To produce the brain-rescued line, we used the tetracycline transactivator (tTA) system, which requires two transgenes for expression of the target gene Bmal1 (Fig. 2A) (14, 15). We used the promoter sequence of Scg2, which is expressed in brain and enriched in the SCN (16), to drive expression of the tetracycline transactivator (tTA) (13). The tTA protein binds to the tetracycline operator (tetO) sequence and drives expression of downstream hemagglutinin (HA)–tagged Bmal1 (Bmal1-HA) cDNA. Doxycycline (Dox) inhibits tTA binding to the tetO promoter, which halts expression of Bmal1-HA. In situ hybridization showed strong, specific expression of Bmal1-HA in Scg2::tTA × tetO::Bmal1-HA double transgenic mice only (Fig. 2B) (13). The pattern of expression observed is consistent with high Scg2 expression in the SCN. HA-tagged protein at the correct molecular weight for BMAL1 (∼69 kD) was produced specifically in double transgenic mouse brain extracts (Fig. 2C), and HA-tagged BMAL1 was shown to be functional by Per1::luciferase reporter gene assays (fig. S2) (13). The double transgenic mice were crossed onto a Bmal1–/– background to create brain-rescued Bmal1–/– mice, and wheel-running experiments were performed as described above (Fig. 2D and figs. S3 to S5).

Fig. 2.

Reversible restoration of circadian rhythms but not activity levels in brain-rescued Bmal1–/– mice. (A) Mice were created to express Bmal1-HA conditionally in brain tissue with the use of the tTA system. (B) In situ hybridization was performed with HA tag or Bmal1 probes on brains from WT, tetO::Bmal1-HA (tetO), or Scg2::tTA × tetO::Bmal1-HA double transgenic (DTg) mice killed at ZT 6 (arrow indicates SCN; scale bar, 1 mm). (C) Western blot showing HA staining in brain, liver, and skeletal muscle protein extracts from WT or DTg mice killed at ZT 12 (arrow indicates correct size of BMAL1). (D) Representative wheel-running activity records from one Bmal1–/– mouse and two brain-rescued Bmal1–/– (DTg; Bmal1 KO) mice. After 3 weeks in DD (Pre), mice were administered Dox for 2 weeks (Dox, highlighted yellow) and then spent an additional 3 weeks without Dox (Post). (E) Brain-rescued mice (n =10) display a free-running period of 22.8 hours (Pre) and 22.6 hours (Post) when Bmal1 is expressed; these values are significantly different from those of WT and DTg groups (*significant effect of genotype, GLM ANOVA). Activity levels of KO and DTg KO mice were significantly reduced relative to WT. Amplitude of circadian rhythm was significantly different in all genotypes relative to WT, and a simultaneous loss of rhythm and decrease in amplitude were observed in DTg KO mice during Dox treatment (†significant effect of time interval). Graphs represent means ± SEM.

Adult (≥8 weeks old) brain-rescued Bmal1–/– mice exhibited a consistent circadian rhythm of behavior in the initial (pre-Dox) DD period, which was completely abolished after 1 to 2 days of Dox administration and then regained during Dox withdrawal. However, the free-running period of brain-rescued mice was about 1 hour shorter than that of wild-type mice (Fig. 2E). This was likely due to the constitutive bioavailability of BMAL1 protein and/or the lack of peripheral tissue feedback to the SCN (fig. S6). In support of this idea, Rev-Erbα–/– mice express Bmal1 in the SCN at consistently high levels and exhibit shortened period length (17). In contrast to the restoration of circadian rhythmicity in brain-rescued mice, both amplitude and activity levels were significantly lower than that seen in wild-type mice (Fig. 2E). Thus, brain-rescued mice exhibit restored circadian rhythms of behavior, but their locomotor activity is still impaired.

Because Bmal1 is highly expressed in muscle, we investigated whether muscle-specific rescue might restore activity levels in Bmal1–/– mice. We produced muscle-specific Bmal1 transgenic mice with the use of a DNA construct consisting of human α-actin–1 (Acta1) promoter sequence positioned upstream of Bmal1-HA (Fig. 3A) (13). HA-tagged protein was specifically detected in transgenic muscle extracts (Fig. 3B) (13). Adult muscle-rescued Bmal1–/– mice did not express circadian rhythmicity of activity (Fig. 3, C and D); however, their level of locomotor activity was not significantly different from that of wild-type mice (Fig. 3D).

Fig. 3.

Muscle-rescued mice exhibit restored activity level but not circadian rhythms. (A) Muscle-specific Bmal1 Tg mice were created by fusing the Acta1 promoter sequence to Bmal1-HA. (B) Western blot shows HA staining in brain, liver, and skeletal muscle protein extracts from WT or Tg mice killed at ZT 12 (arrow indicates correct size of BMAL1). (C) Representative wheel-running activity records are shown from WT, Tg, Bmal1 KO, and muscle-rescued (Tg; Bmal1 KO) mice. (D) Muscle-rescued mice (n = 6) are arrhythmic in DD with significantly reduced amplitude of rhythm (**P < 0.01, one-way ANOVA) but display activity levels that are not significantly different from those of WT mice. Graphs show means ± SEM (*significant effect of genotype, GLM ANOVA).

We also found that at 4 to 6 months of age, the Bmal1–/– and brain-rescued mice weighed significantly less than did wild-type mice. In contrast, the body weight of muscle-rescued mice was restored to a level not significantly different from that of wild-type mice (Fig. 4A) (13). Only 75% of brain-rescued mice survived to the end of the experiment, whereas 100% of muscle-rescued mice survived. These results suggest that BMAL1 function in muscle is important for activity as well as for body weight maintenance and longevity. In addition, bone phenotypes were examined by Alizarin Red stain; both brain- and muscle-rescued mice showed significant tendon calcification similar to that seen in Bmal1–/– mice (Fig. 4B). This suggests that Bmal1-HA was not expressed in bone in either line and that the calcification observed in Bmal1–/– mice was not improved by restoring BMAL1 expression in muscle or brain. Thus, three distinct patterns of rescue could be observed in these mice, relating to (i) circadian activity rhythms, (ii) activity level and body weight, and (iii) tendon calcification.

Fig. 4.

Effects of tissue-specific Bmal1 expression on body weight and tendon calcification. (A) Brain-rescued mice and KO mice in both lines have significantly reduced body weight, whereas muscle-rescued mice exhibit body weight similar to that of WT mice (graphs represent means ± SEM; **P < 0.01, ***P < 0.001, one-way ANOVA). (B) Photographs of Alizarin Red–stained hindlimbs from WT, KO, muscle-rescued, and brain-rescued KO mice. Arrows indicate calcaneal tendon calcification in all but WT mice.

Unlike the BAC transgenic line, the brain and muscle transgenic lines were designed to constitutively express BMAL1-HA. To verify this, we measured similar levels of Bmal1 mRNA and BMAL1-HA protein at normal peak and trough times in the brain and muscle transgenic lines (figs. S6 and S7) (13). We then examined mRNA levels of the key BMAL1 target genes Per1 and Per2 (6). Relative to wild-type and brain double transgenic mice, Bmal1–/– mice exhibited consistently low expression levels of Per1 in the SCN (fig. S7). In contrast, the brain-rescued mice had increased amplitude of Per1 expression, although peak levels remained significantly lower than those of wild-type mice (fig. S7). Per2 expression was measured in both muscle and liver from the brain and muscle transgenic lines at normal peak time for Per2 (ZT 12) (fig. S8) (13). Per2 in muscle of Bmal1–/– mice was significantly reduced to below 50% of wild-type levels at ZT 12. This decreased expression was restored to wild-type levels in the muscle-rescued mice but not in the brain-rescued mice. These data suggest that the presence of BMAL1 is important for proper expression of Per1 genes in brain and muscle tissue (but not liver; see fig. S8). Both Rev-Erbα and Dbp exhibited substantial down-regulation in liver and muscle of Bmal1–/– mice at ZT 12 and showed increased expression only in muscle of muscle-rescued mice (fig. S8).

We have shown that all phenotypes of Bmal1–/– mice are alleviated only when Bmal1 is rescued ubiquitously, whereas different parameters of behavioral activity (circadian rhythm and activity level), body weight, and gene expression can be rescued separately by distinct spatial expression patterns of Bmal1. Genome-wide profiling experiments suggest that ∼10% of the transcriptome is under circadian regulation; however, the majority of these cycling transcripts are tissue-specific (1822). Our results are consistent with this tissue-specific diversity of circadian expression and further suggest that core circadian clock components may play distinct roles in different tissues, perhaps in addition to their function in regulating circadian rhythms. The restoration of circadian activity rhythms in brain-rescued Bmal1–/– mice is consistent with previous SCN transplant studies in rodents (23, 24). However, the transgenic approach used here has the advantages of preserving the anatomical integrity of the brain as well as allowing the conditional manipulation of the rescue via Dox treatment. The use of tissue-specific and conditional regulation of circadian clock gene expression should be a valuable method for understanding the molecular-, cellular-, and systems-level regulation of circadian rhythms in mammals.

Supporting Online Material

www.sciencemag.org/cgi/content/full/314/5803/1304/DC1

Materials and Methods

SOM Text

Figs. S1 to S8

References

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