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Demonstration of Genetic Exchange During Cyclical Development of Leishmania in the Sand Fly Vector

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Science  10 Apr 2009:
Vol. 324, Issue 5924, pp. 265-268
DOI: 10.1126/science.1169464

Abstract

Genetic exchange has not been shown to be a mechanism underlying the extensive diversity of Leishmania parasites. We report here evidence that the invertebrate stages of Leishmania are capable of having a sexual cycle consistent with a meiotic process like that described for African trypanosomes. Hybrid progeny were generated that bore full genomic complements from both parents, but kinetoplast DNA maxicircles from one parent. Mating occurred only in the sand fly vector, and hybrids were transmitted to the mammalian host by sand fly bite. Genetic exchange likely contributes to phenotypic diversity in natural populations, and analysis of hybrid progeny will be useful for positional cloning of the genes controlling traits such as virulence, tissue tropism, and drug resistance.

Parasitic protozoa of the genus Leishmania cause a spectrum of human diseases that pose serious public health challenges for prevention, diagnosis, and treatment. The diversity of Leishmania species, with more than 20 currently recognized, is thought to have arisen by gradual accumulation of divergent mutations rather than by sexual recombination. Tibayrenc et al. (1) have reported strong linkage disequilibrium in several Leishmania species and proposed that these parasites are essentially clonal. This notion must be reconciled, however, with the accumulating examples of naturally occurring strains that share genotypic markers from two recognized species and thereby provide circumstantial evidence for sexual recombination (24). Genetic exchange has been documented for the other trypanosomatids that cause human disease. Hybrid genotypes were observed in tsetse flies during cotransmission of two strains of Trypanosoma brucei (5) and in mammalian cells after coinfection with two clones of Trypanosoma cruzi differing in drug-resistance markers (6). Using drug resistance markers, we provide evidence for genetic exchange in Leishmania major and discuss the implications of these findings to Leishmania biology and experimental analysis.

One parental clone, LV39c5(HYG), was derived from strain LV39 clone 5 (MHOM/SU/59/P) and was heterozygous for an allelic replacement of the LPG5A on chromosome 24 by a hygromycin B–resistance cassette (LPG5A/LPG5A::ΔHYG) (7). The second parental clone, FV1(SAT), was derived from NIH Friedlin clone V1 (MHOM/IL/80/FN) and bore a heterozygous nourseothricin–resistance (SAT) marker, integrated along with a linked firefly luciferase (LUC) reporter gene into one allele of the ∼24 rRNA cistrons located on chromosome 27 (8) (+/SSU::SAT-LUC). These strains were chosen as they are phenotypically identical to their respective parental wild-type (WT) virulent L. major; whereas the markers were chosen because they are functionally independent (9). The target gene modifications were chosen because they caused no effect on normal growth in vitro or in mouse infections (10), and epistatic interactions were not anticipated between these alleles.

Multiple attempts to generate hybrid parasites resistant to both antibiotics during in vitro co-culture of the parental lines were unsuccessful (11). The parental clones were tested for their ability to generate parasites resistant to both drugs during coinfection in the sand fly. The growth of each parental line in Phlebotomus duboscqi, a natural vector of L. major, is shown in fig. S1. Promastigotes of each parent survived the initial period of blood-meal digestion and excretion (days 1 to 6) and underwent metacyclogenesis at a comparable frequency (20 to 60%), although the FV1(SAT) parent established and maintained a higher intensity of infection by a factor of 3 to 4. The parental clones were tested for their ability to generate doubly drug–resistant parasites during coinfection in the sand fly. Flies were fed through a membrane on mouse blood containing 3 and 1 × 106/ml of the LV39c5(HYG) and FV1(SAT) lines, respectively, each obtained from log-phase cultures and extensively washed to remove antibiotics. A total of 102 flies from four independent coinfection experiments were dissected 13 to 16 days postinfection; at this time, they harbored mature infections with an average of 39,400 ± 14,700 promastigotes per midgut. Flies cannot be maintained under aseptic conditions, and more than half of the cultures established from the midgut parasites were lost to fungal contamination during the subsequent 1 to 2 weeks of culture. In the remaining cultures, 12 (26%) grew out promastigotes that were resistant to both drugs. Clonal lines were generated from nine of the doubly drug–resistant populations, and the genotypes and phenotypes of one or two clones from each culture were determined (summarized in Table 1).

Table 1.

Summary of phenotype and genotype data for parental and progeny clones. Hybrid lines nomenclature: experiment number.fly number.clone name, and for 6.14.F9 and 6.16.E8, experiment number.ear lesion number.clone name. Virulence profile: fast (f) or slow (s). Maxicircle inheritance: F, FV1(SAT) SNP pattern; L, LV39c5(HYG) SNP pattern. Chromosomal analysis: SEQ, ratio of parental SNP peaks by sequence analysis; CAPS, cleaved and amplified polymorphic site analysis; and H, hybrid. The six chromosomes are 2, 7, 21, 25, 35, and 36. Chromosome 31 is tetrasomic.

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Polymerase chain reaction (PCR) tests with primers specific for the parental markers showed that all doubly drug–resistant clones tested contained both the HYG and SAT drug-resistance genes (Fig. 1A, Table 1, and table S3). Controls showed that the marker loci had not rearranged during hybrid formation but had maintained their location within the LPG5A or SSU rRNA loci, respectively (fig. S2). These data indicated that the doubly drug–resistant clonal lines were genetic hybrids. To confirm that hybrid formation was compatible with transmission to the mammalian host, coinfected sand flies were allowed to bite and to induce lesions in BALB/c mice. Two doubly drug–resistant clonal lines, designated 6.16.E8 and 6.14.F9, were recovered from the eight dermal lesions examined and were found to be similar to those directly recovered from insects, as discussed below (Table 1). Co-injection of both parental lines into mouse ears by needle never led to the recovery of doubly drug–resistant parasites from the 20 dermal lesions examined, each containing >2.4 × 107 amastigotes, which suggested that the hybrids selected after transmission by the coinfected sand flies were generated in the fly and not in the mammalian host.

Fig. 1.

Genotyping of hybrid Leishmania. (A) PCR for parental selectable drug markers SAT and HYG. Samples are F, FV1(SAT); L, LV39c5(HYG); M, 1 kb plus marker (Invitrogen, Carlsbad, CA); -, no template control, 1, 1.10.B12; 2, 1.10.D9; 3, 4.3.G12; 4, 4.3.A12; 5, 1.14.E10; 6, 5.12.D9; 7, 5.12.F11; 8, 1.14.B11; and 9, 5.22.10. (B) SNP-CAPS analysis for loci on chromosomes 7, 25, and 35. F, L and M are as in (A). Mix a, b, and c, parental templates mixed in different ratios (1:1, 3:1, or 1:3 F:L, respectively). DNA content analysis showed that hybrids 5 and 8 are 3n (marked by an asterisk), and the remainder shown are 2n progeny. (C) SNP-CAPS analysis of maxicircle. Digestion with Bfa I of the ND5–divergent region PCR product is shown. Hybrids tested are 1, 4.7.A3; 2, 4.17.A3; 3, 4.9.E6; 4, 4.17.F4; 5, 1.10.B12; 6, 1.10.D9; 7, 4.3.G12; 8, 4.3.A12; 9, 1.14.E10; 10, 5.12.D9; 11, 5.12.F11; 12, 1.14.B11; and 13, 5.22.10.

We examined the segregation of loci not linked to chromosomes 24 and 27, the location of the HYG and SAT markers, respectively. We used single-nucleotide polymorphisms (SNPs) developed from comparisons of the terminal ∼30-kb chromosomal regions encompassing the SCG genes located on L. major chromosomes 2, 7, 21, 25, 31, 35, and 36 in the WT parent of LV39c5(HYG). SCGs make up a family of polymorphic telomeric galactosyltransferases (12), but genes internal to these showed SNPs occurring at an overall frequency of ∼0.15%, consistent with other estimates of strain variation (13, 14). For each chromosome, SNPs from one to two loci, located from 8.5 to 23 kb inwards of the telomeric SCGs (12) (tables S1 and S2), were analyzed by a combination of SNP-CAPS (15) [SNP genotyping combined with cleaved amplified polymorphic site analysis (CAPS)] and/or direct sequencing. Each parent was homozygous for every marker tested, and all 18 progeny showed clearly that they had inherited both parental alleles (Fig. 1B and Table 1). This provides evidence that each progeny clone inherited a full set of chromosomes from each parent and were thus full genome hybrids.

In 7 out of 18 hybrid progeny clones, the relative ratio of the parental alleles seen in SNP-CAPS digestions differed from the expected 1:1. Instead, in each case the most intense bands or sequencing trace peaks were those associated with the LV39c5 parent, a finding seen at all loci and in all lines tested (Fig. 1B, fig. S3, and Table 1). One possibility is the occurrence of triploid offspring, bearing two chromosomal complements from one parent (LV39) and one from the other (FV1). The DNA contents of the progeny clones were measured relative to those of the parents by flow cytometry, and the profiles compared with 2n and 4n lines studied previously (16). Although the parents and most hybrids showed 2n DNA contents, the seven hybrids detected above showed 3n DNA content (Fig. 2 and Table 1); intermediate DNA content was not observed. Thus, although all doubly drug–resistant lines were full genome hybrids, a significant fraction appeared to be triploid rather than diploid and had inherited two genomic complements from the LV39 and one from FV1 parents. Similarly, for four of the 2n lines (1.16. A1, 1.16.C4, 5.12.D9, and 5.12.F11), the segregation of chromosome 31 markers appeared to differ from the expected 1:1 ratio, with the LV39c5 parent again predominating in sequencing and/or SNP-CAPs analysis of the hybrids (Table 1). This may arise from the finding that Leishmania chromosomes are occasionally aneuploid (17), which includes tetrasomy of Leishmania chromosome 31 in the parental lines studied here. Potentially, tetrasomy may affect mitotic inheritance and segregation, as seen in autotetraploid or allotetraploid species (18). This was not pursued further in this study.

Fig. 2.

DNA contents of parental and 2n and 3n progeny clones. DNA content was measured in log phase cells by flow cytometry after staining RNase-treated permeabilized cells with propidium iodide. (A) Parental lines: FV1(SAT), red, and LV39c5(HYG), green. (B) Representative 2n (green, 4.3.G12 and 1.10.D9) and 3n (blue, 1.14.E10 and 4.9.C8) progeny. M1 refers to cells in the G1/G0 phase of the cell cycle and M2 refers to cells in G2/M phase. Small differences in the M1/M2 ratio reflect minor differences in cellular growth rate and growth phase and are not significant nor found in other studies of these lines.

SNP markers were identified to determine the inheritance of mitochondrial maxicircle formed of kinetoplast DNA (kDNA) (Fig. 1C, Table 1, and tables S1 to S3). In contrast to the chromosomal DNA, maxicircle markers demonstrated clear and consistent uniparental inheritance, with 6 of the progeny clones having maxicircle kDNA exclusively from the LV39c5(HYG) and 12 inheriting maxicircle kDNA exclusively from FV1(SAT) (Table 1 and Fig. 1C). These markers allowed us to establish that two clonal lines arising from the same fly that had identical nuclear markers were in fact different (4.9.C8/C6 and 4.3.A12/G12) (Table 1). kDNA minicircles were not examined.

Several studies were undertaken to compare the progeny clones for parental phenotypic traits. Metacyclic promastigotes of the FV1 line react with monoclonal antibody (mAb) 3F12, which recognizes the abundant surface lipophosphoglycan (LPG) and other phosphoglycans expressing mono–β-galactose–modified repeat units terminating with β(1,2)arabinose residues. The same antibody fails to bind to LV39c5 metacyclics, owing to differences in the β-galactose chain length (19). Metacyclic promastigotes purified from all progeny clones displayed strong reactivity with mAb 3F12, as determined by surface agglutination of live parasites (fig. S4A). Thus, the mono-galactosylated 3F12 trait appeared to be inherited as a dominant trait. Another dominant trait is the “clumpy” appearance of LV39c5(HYG) when grown in standard culture medium, in contrast to FV1(SAT), which grows as individual cells. All hybrid offspring appeared clumpy (fig. S4B).

It is noteworthy that the parental clones differ in a virulence trait defined as the time required for the emergence of lesions in susceptible BALB/c mice (Fig. 3). After subcutaneous foot-pad inoculation of 104 metacyclic promastigotes, purified in each case from stationary cells freshly transformed from lesion amastigotes, five of the progeny clones displayed lesion progression as fast or faster than the FV1(SAT) parent, whereas another five of the progeny showed lesion development as slow or slower than the LV39c5(HYG) parent. These data indicate that despite the low nucleotide heterozygosity typically found in L. major (14), there is sufficient variation in one or both parental clones for distinct virulence phenotypes to emerge. The most parsimonious explanation is that either “slow” or “fast” growth is inherited as a dominant trait and that one or both parental clones are heterozygous for the gene(s) controlling this trait.

Fig. 3.

Lesion formation in BALB/c mice by parental and hybrid progeny clones. Mice were infected in the hind foot pad by subcutaneous inoculation of 104 metacyclic promastigtoes. Results are representative of three independent experiments.

All five triploid lines tested exhibited the “slow virulence” trait, which occurred in only one out of eight of the diploid lines. There may be a reduction in virulence associated with polyploidy that accounts for the failure to detect polyploid lines in field isolates. In contrast, no significant association was seen with the maxicircle genotype and virulence or ploidy (Table 1).

Leishmania can undergo genetic exchange during growth and development in the sand fly vector and can transmit infectious-stage hybrid progeny to a mammalian host. Based on the analysis of 18 hybrid clones representing a minimum of 11 independent crosses, the findings argue for inheritance of at least one full set of chromosomes from each parent, accompanied by independent, uniparental inheritance of the maxicircle kDNA derived from one parent. The inheritance patterns of nuclear DNA fit a Mendelian model of meiosis of the parental strains followed by fusion of the haploid cells. However, alternative mechanisms, modeled after the tetraploid sexual cycle described in Saccharomyces cerevisiae (20), could involve fusion of parental diploid cells, followed by meiosis and intracellular fusion of haploid nuclei (21, 22). Triploid offspring have also been seen in trypanosome crosses and have been attributed to incomplete meiotic division and fusion of haploid and diploid nuclei (fig. S5) (21, 23). In trypanosomes as well, maxicircle kDNA was initially thought to be inherited uniparentally; however, later studies showed it to be inherited biparentally, but subsequently to segregate out during mitosis, leading to fixation (24). It is possible that mixed maxicircle genotypes might have also been present in earlier generations of the Leishmania crosses than were examined here.

The frequency of genetic exchange involving these two parental clones would appear to be rare (∼2.5 × 10–5 or less, after correcting for recovery of only doubly drug–resistant offspring). Consistent with this, most clonal lines from a single infected fly were identical, although two flies yielded offspring with different maxicircle genotypes, which suggested two independent crossing events. Whether the frequency observed in the FV1 × LV39c5 cross here is typical for other Leishmania strains or species remains to be determined. The low frequency agrees with the general sense that gene exchange must occur rarely, as deduced from observed heterozygosities and linkage disequilibrium in natural populations (1).

Despite the infrequency of gene exchange experimentally or in nature, there are many examples of hybrid genotypes observed in field isolates involving most Leishmania species (24, 2527). Potentially, these hybrids arose from rare “mating” events, yielding offspring with a strong selective advantage, such as seen in Toxoplasma gondii (28), and suggested by the clonal propagation of an emergent hybrid mucosal strain in Peru (3). Given the rarity with which mixed infections in flies are likely to occur, in conjunction with the low frequency of hybridization that we have observed in coinfected flies, any successful new genotype would be expected to propagate clonally.

Although rare in nature, the frequency of experimental hybrid formation is sufficient to enable its use as an experimental tool. Our studies show segregation of “virulence traits” in the FV1 × LV39c5 crosses studied here, and through positional cloning, the genes responsible may be identified. Future studies will explore the possibilities of carrying out backcrosses, as well as crosses between species, and of developing SNP tools for genetic linkage analysis.

Supporting Online Material

www.sciencemag.org/cgi/content/full/324/5924/265/DC1

Materials and Methods

Figs S1 to S5

Tables S1 to S3

References

References and Notes

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