Functional Characterization of the Antibiotic Resistance Reservoir in the Human Microflora

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Science  28 Aug 2009:
Vol. 325, Issue 5944, pp. 1128-1131
DOI: 10.1126/science.1176950

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  • RE: Could it be possible that in vitro culture technique used confound the detection of Resistance gene?
    • Dinesh Bhandari, Health Researcher, Public Health research laboratory, Institute of medicine.

    As reported in this paper, "the proportion of identified transposases derived from the culturable aerobic isolates (90%) was significantly higher than those derived from metagenomic sampling". Could it be possible the culture technique (the content of media) used has got something to do with the higher detection of inserts encoding antibiotic resistance gene compared to the detection of resistance gene from metagenomic data.
    Naturally, only those genes that are expressed in the host used i.e, E. coli in this can can be detected. Also, various antibiotics supplemented in the growth of host organism used to express the vector integrated with metagenomic DNA fragment interfere with the actual presentation of resistance gene. Given, these limitation still? Would there be possibility of over-representation of antibiotic resistome detection from same organism if we compare the two techniques i.e., direct culturable organism sequencing from their in vitro culture and functional metagenomic clone sequencing from their in vitro culture given the content of two media used are more or less similar.

    Competing Interests: None declared.
  • Are Human Microflora Influenced by Environmental Exposure to Antibiotics?

    The Report by M. O. Sommer et al. describes an extensive array of antibiotic resistance genes found in the human microflora ("Functional characterization of the antibiotic resistance reservoir in the human microflora," 28 August 2009, p. 1128), including the discovery of 95 unique inserts containing functional antibiotic resistance genes. More than one out of four of these genes encode beta-lactamases that inactivate b...

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    Competing Interests: None declared.

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