Research Article

Reactome Array: Forging a Link Between Metabolome and Genome

See allHide authors and affiliations

Science  09 Oct 2009:
Vol. 326, Issue 5950, pp. 252-257
DOI: 10.1126/science.1174094

You are currently viewing the abstract.

View Full Text

Log in to view the full text

Log in through your institution

Log in through your institution

This article has been retracted. Please see:


We describe a sensitive metabolite array for genome sequence–independent functional analysis of metabolic phenotypes and networks, the reactomes, of cell populations and communities. The array includes 1676 dye-linked substrate compounds collectively representing central metabolic pathways of all forms of life. Application of cell extracts to the array leads to specific binding of enzymes to cognate substrates, transformation to products, and concomitant activation of the dye signals. Proof of principle was shown by reconstruction of the metabolic maps of model bacteria. Utility of the array for unsequenced organisms was demonstrated by reconstruction of the global metabolisms of three microbial communities derived from acidic volcanic pool, deep-sea brine lake, and hydrocarbon-polluted seawater. Enzymes of interest are captured on nanoparticles coated with cognate metabolites, sequenced, and their functions unequivocally established.

  • * These authors contributed equally to this work.

  • These authors contributed equally to this work.

View Full Text

Stay Connected to Science