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Meiotic Recombination Provokes Functional Activation of the p53 Regulatory Network

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Science  04 Jun 2010:
Vol. 328, Issue 5983, pp. 1278-1281
DOI: 10.1126/science.1185640

Abstract

The evolutionary appearance of p53 protein probably preceded its role in tumor suppression, suggesting that there may be unappreciated functions for this protein. Using genetic reporters as proxies to follow in vivo activation of the p53 network in Drosophila, we discovered that the process of meiotic recombination instigates programmed activation of p53 in the germ line. Specifically, double-stranded breaks in DNA generated by the topoisomerase Spo11 provoked functional p53 activity, which was prolonged in cells defective for meiotic DNA repair. This intrinsic stimulus for the p53 regulatory network is highly conserved because Spo11-dependent activation of p53 also occurs in mice. Our findings establish a physiological role for p53 in meiosis and suggest that tumor-suppressive functions may have been co-opted from primordial activities linked to recombination.

The p53 gene family mediates adaptive responses to genotoxic stress (13) and is broadly conserved (4, 5). It is widely accepted that the p53 regulatory network is generally compromised in human cancers, but several lines of evidence indicate that during evolution, animals rarely lived long enough to experience cancer (6, 7). Therefore, tumor-suppressive functions associated with p53 were probably derived from primordial activities that are poorly understood. We used Drosophila to investigate physiological properties of this network because, in this organism, a single p53 member exists and, like its mammalian counterparts, it coordinates stress responses and promotes genome stability (812).

We constructed transgenes (fig. S1) that place green fluorescent protein (GFP) under the control of an enhancer taken from sequences upstream of the Drosophila reaper (rpr) locus, which includes a p53 consensus binding site (13, 14). Two transgenic lines were produced and designated as “p53Rps” for p53 reporter strains. One strain produces nuclear localized GFP (p53R-GFPnls), and the other does not (p53R-GFPcyt).

Ionizing radiation (IR) leads to DNA damage and activates p53 in various model systems. Therefore, we exposed p53Rps transgenic embryos to IR and followed GFP expression by time-lapse live imaging. GFP was observed as early as 70 min after exposure to IR and was prominent at 180 min in virtually all embryos (Fig. 1A and movie S1). p53Rps expression was not observed in unirradiated embryos, nor was it observed in flies lacking p53 or chk2 (Fig. 1B), its upstream activating kinase (15). To confirm that damaged DNA is responsible for activation, we also observed induction of GFP in response to ultraviolet radiation and injected DNA fragments (fig. S2). Thus, the p53Rps reporters serve as authentic proxies that enable us to monitor p53 activation in live animals in real time.

Fig. 1

Transgenic reporters as in vivo surrogates for p53 activation. (A) Live imaging of p53R-GFPnls expression after IR challenge at indicated time points (see also movie S1). Autofluorescent yolk material is marked with white dotted lines. Note that IR challenge did not interfere with germ-band retraction (white arrowheads). (B) Stimulus-induced p53R-GFPnls expression was seen in WT animals, but not in p53−/− or chk2−/− animals. Scale bars, 10 μm.

We surveyed reporter activity throughout development and found little or no evidence for unstimulated expression, but, surprisingly, transient p53Rps expression was observed in germline precursors of all females. Activity was localized in regions 2a and 2b of the germarium and was absent beyond region 3 and in all egg chambers (Fig. 2). We also examined p53Rps in the germaria of p53−/− or chk2−/− animals and found no expression (Fig. 2B and fig. S4). Together, these observations indicate that a pulse of p53 activity occurs in oocyte precursors.

Fig. 2

Meiotic recombination instigates programmed activation of p53 in the Drosophila female germ line. (A) Schematic illustration of female germarium in the Drosophila ovary. Region 1 contains germline stem cells (GSC) and their progeny, cystoblasts (CB). Cysts in regions 2a and 2b initiate meiosis and further develop into egg chambers in region 3. Regions in the germarium are visualized with the use of immunostaining for hu-li tai shao (HTS) protein. (B and C) Genotypes as indicated were stained for GFP (green) and HTS (red). Animals carry p53R-GFPnls or p53R-GFPcyt in (B) or (C), respectively. Stereotyped GFP activation in region 2 is indicated by solid arrowheads; the absence of staining is indicated with open arrowheads. Scale bars, 10 μm. (D) Percentage of germarium with p53R-GFPcyt expression in regions 2a and 2b. Means ± SDs (error bars) from at least three independent trials are plotted, and sample size is denoted within parentheses.

Meiotic recombination is initiated in regions 2a and 2b by Spo11 (also known as mei-W68), a topoisomerase that generates the DNA double-strand breaks (DSBs) needed for strand exchange (16). Therefore, we tested p53Rps activity in germaria lacking Spo11. Activation of p53 in regions 2a and 2b was absent in spo11−/− ovaries (Fig. 2C). Furthermore, DSBs introduced by IR were sufficient to restore p53Rps activity in regions 2a and 2b of spo11−/− germaria (Fig. 2D). Thus, DSBs formed during the initiating steps of meiotic recombination provoke activation of p53.

Flies lacking p53 are viable and fertile. Therefore, the gene does not exert essential functions needed for gametogenesis or proper chromosome segregation (table S2). To test whether meiotic recombination frequencies were altered in p53−/− animals, we assayed three distinct p53 alleles in trans-allelic combinations for meiotic exchange frequencies. Reduced crossover rates, ranging from 21 to 54%, were observed across all intervals (Fig. 3A and table S1).

Fig. 3

Functions of p53 activity during meiosis. (A) Meiotic recombination frequency in WT and p53−/− flies. Recombination frequency is reduced in all p53 transallelic combinations when compared with the wild type (Canton S and yw) at all three intervals. Arrows show expected frequencies (31). (B) Immunostaining of GFP in p53R-GFPcyt animals that are WT or rad54 mutants. Persisting GFP expression beyond region 2 (white dotted circle) is observed in rad54 mutants (denoted by red arrowheads). Yellow dotted circle, region 3; yellow dotted line, egg chambers. Scale bars, 10 μm. (C) The incidence of p53Rps expression was quantified in region 3 and in stage two to eight egg chambers, as indicated. Error bars denote SDs from three trials. (D) Images of egg chambers stained with DAPI (4′,6′-diamidino-2-phenylindole) from indicated genotypes. Red stars mark individual nurse cell nuclei. Scale bars, 10 μm. (E) Distribution of the number of nurse cell nuclei per egg chamber (nuclei/EC). Single-gene mutants show 15 nuclei/EC, but p53−/−,rad54AA/RU ovaries exhibit a broad distribution, ranging from 9 to 40 nuclei/EC, which is restored to normal in spo11−/−,rad54AA/RU,p53−/− animals (blue group).

To understand the function of p53 during meiotic recombination, we monitored p53 activity in mutants defective for proper meiosis. Rad54 is required to properly resolve DNA crossovers, and in the germaria of rad54 (okra) mutants, unrepaired DNA breaks abnormally persist (17). In such animals, we found persistent activation of p53 beyond region 2 (Fig. 3, B and C), as indicated by p53Rps expression in region 3 of the germarium and later-staged egg chambers, where a substantial increase in the incidence of reporter activation was observed (Fig. 3C). To test whether persistent p53 activation is functionally relevant, we examined animals doubly mutated for p53 and rad54. p53−/−,rad54AA/RU females were sterile, despite the fact that corresponding trans-allelic combinations of the single-gene mutants were fertile (see methods for allele descriptions). Furthermore, severe oogenesis defects, which are not seen in single mutants, were evident in double mutants, including abnormal numbers of nurse cell nuclei (Fig. 3D) and shortened egg lengths (fig. S5). To test whether genetic interactions between p53 and rad54 were instigated by meiotic recombination, we created spo11,p53,rad54 triple mutants. In these animals, fertility and normal nurse cell numbers were restored (Fig. 3, D and E), and defects in egg length were suppressed (fig. S5). Hence, genetic interactions between p53 and rad54 required the action of spo11. Furthermore, these results indicate that failure to properly resolve meiotic recombination can lead to sustained and functionally relevant p53 activity.

To determine whether activation of p53 by meiotic recombination is conserved, we examined mouse testes with antibodies that specifically detect phosphorylated p53 at Ser15 (18). In seminiferous tubules from wild-type (WT) animals, p53 was transiently activated in early spermatocytes (Fig. 4, A and B). On the basis of nuclear morphology (described in supplemental methods), staining appeared to peak between the leptotene and zygotene stages and disappeared after the early pachytene stage. This pattern is consistent with earlier studies on Spo11 activity (19) and p53 promoter–driven chloramphenicol acetyltransferase transgenes in this tissue (20). Staining for phospho-p53 (Ser15) was absent from testes of Spo11-deficient mice (Fig. 4, B and C), where spermatocytes survive through the leptotene and zygotene stages (21). Therefore, we can exclude cell loss as a reason for the absence of signal, and, as in flies, meiotic recombination is necessary to provoke p53 activation.

Fig. 4

Spo11-dependent p53 activation is conserved in the mouse. (A to C) Immunostainings for phospho-Ser15 p53 in paraffin sections from WT [(A) and (B)] and Spo11 knockout (C) mouse testes. DAB (3,3′-diaminobenzidene) (A, brown) and fluorescent [(B) and (C), green] detection methods show that transient activation of p53 in meiotic cells is absent in Spo11-deficient testes. DNA staining (hematoxylin or Hoechst) is shown in blue. (B′ and C′) Signal without Hoechst counterstain. Insets in (A) and (B) show nuclear localized staining of Ser15-p53 under comparable magnifications. Scale bars, 100 μm (A); 10 μm [(B) and (C), insets in (A)].

We demonstrate that a defining step in sexual reproduction, meiosis, signals a programmed burst of p53 activation. This activity is stimulated by the action of Spo11 in Drosophila females (recombination does not occur in males) and in mice. In flies, activation requires the kinase chk2 [which also has roles later in oogenesis (22)] but appears to be independent of the ATM and ATR kinases (fig. S3), suggesting that alternative transducers could mediate Spo11-dependent activation of p53.

In mice (Fig. 4), potential functions of Spo11-mediated activation of p53 are indicated by altered kinetics of gametogenesis in p53-deficient mice (23, 24), as well as giant-cell degenerative syndrome in the testes of p53-deficient males (25) and implantation defects in females (26). Additional layers of complexity or redundant activities conferred by the p63 and p73 paralogs (27, 28) could obscure conserved functions, because recombination appeared normal without p53 (29). Nevertheless, these findings raise the possibility that the act of recombination during meiosis may have been an intrinsic primordial stimulus that shaped ancestral features of the p53 regulatory network. Future studies could elucidate whether p53 directly affects crossover reactions (30) or imposes quality control on selected gametes.

Supporting Online Material

www.sciencemag.org/cgi/content/full/328/5983/1278/DC1

Materials and Methods

Figs. S1 to S5

Tables S1 and S2

References

Movie S1

References and Notes

  1. Materials and methods are available as supporting material on Science Online.
  2. We thank S. Keeney for helpful discussions and reading of the manuscript and A. Ali, A. D’Brot, and A. Olivo for assisting experiments. We also thank the following individuals, laboratories, or facilities for helpful discussions, access to instruments, or providing reagents: S. Barolo, M. Buszczak, D. Castrillon, O. Cleaver, K. Hamra, J. Maines, D. McKearin, T. Schupbach, Y. Rong, J. Shelton, T. Wilkie, R. Bachoo, T. Mashimo, B. Thaden, Live Cell Imaging Facility (Univ. of Texas Southwestern), Bloomington Stock Center (Indiana Univ.), Developmental Studies Hybridoma Bank (Univ. of Iowa). This work was supported by the National Institute of General Medical Sciences (grant R01GM072124) and the National Institute on Alcohol Abuse and Alcoholism (grant R01AA017328).
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