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Btbd7 Regulates Epithelial Cell Dynamics and Branching Morphogenesis

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Science  30 Jul 2010:
Vol. 329, Issue 5991, pp. 562-565
DOI: 10.1126/science.1191880

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  • RE: Correction to: “Btbd7 regulates epithelial cell dynamics and branching morphogenesis”
    • Kenneth M. Yamada, NIH Distinguished Investigator, NIDCR, NIH
    • Other Contributors:
      • Tomohiro Onodera, Lecturer, Hokkaido University
      • Takayoshi Sakai, Professor and Chairman, Osaka University
      • William P. Daley, Scientific Program Manager, NINDS, NIH
      • Shaohe Wang, Postdoctoral Fellow, NIDCR, NIH
      • Kazue Matsumoto, Biologist, NIDCR, NIH

    In this manuscript analyzing mechanisms of organ branching morphogenesis, we used in situ hybridization with extensive tyramide amplification to examine the site of expression of Btbd7 mRNA in embryonic mouse salivary glands and concluded: “Btbd7 expression by in situ hybridization showed striking concentration around the bottom and lower sides of forming clefts, whereas other salivary epithelial regions showed little or no expression (Fig. 2, A to D, and fig. S4).” This finding suggests that Btbd7 protein would also be present at the same sites. However, in a recent characterization of Btbd7 protein immunolocalization (Daley et al., “Btbd7 is essential for region-specific epithelial cell dynamics and branching morphogenesis in vivo” Development 144: 2200-2211, 2017), we did not observe enhanced local concentration near forming clefts. Because this protein localization failed to coincide with the Btbd7 mRNA localization reported previously in our Onodera et al. (2010) paper, we re-examined its mRNA localization by performing single molecule fluorescence in situ hybridization (smFISH) of salivary gland whole mounts to determine more quantitatively the distribution of Btbd7 mRNA. According to smFISH, Btbd7 mRNA is ubiquitously expressed across the salivary gland epithelium, with no local enrichment (Daley et al., 2017).
    The differences between our reported in situ localization of mRNA at clefts in our previous study and our recent publication may be due to differ...

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    Competing Interests: None declared.