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Tau Reduction Prevents Aβ-Induced Defects in Axonal Transport

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Science  08 Oct 2010:
Vol. 330, Issue 6001, pp. 198
DOI: 10.1126/science.1194653

Abstract

Amyloid-β (Aβ) peptides, derived from the amyloid precursor protein, and the microtubule-associated protein tau are key pathogenic factors in Alzheimer’s disease (AD). How exactly they impair cognitive functions is unknown. We assessed the effects of Aβ and tau on axonal transport of mitochondria and the neurotrophin receptor TrkA, cargoes that are critical for neuronal function and survival and whose distributions are altered in AD. Aβ oligomers rapidly inhibited axonal transport of these cargoes in wild-type neurons. Lowering tau levels prevented these defects without affecting baseline axonal transport. Thus, Aβ requires tau to impair axonal transport, and tau reduction protects against Aβ-induced axonal transport defects.

Amyloid-β (Aβ) peptides, derived from the amyloid precursor protein (APP), and the microtubule-associated protein tau are key pathogenic factors in Alzheimer’s disease (AD). However, the exact mechanisms by which they impair cognitive functions are unknown. Human APP (hAPP) transgenic mice have high Aβ concentrations in the brain and develop aberrant neuronal activity and behavioral deficits (1, 2). Lowering endogenous tau prevents these abnormalities without affecting baseline neuronal functions (1, 2). The mechanisms of this rescue are unknown. Axonal transport is critical for neuronal function and is impaired by Aβ (36). Whether tau also affects axonal transport is controversial (79).

To explore whether tau reduction prevents Aβ-induced defects in axonal transport, we studied axonal transport of mitochondria and the neurotrophin receptor TrkA, whose neuronal distributions are altered in AD (10, 11). Hippocampal neuronal cultures from tau-deficient (Tau−/− or Tau+/−) mice (1) and wild-type (Tau+/+) controls were transfected with plasmids expressing fluorescent markers of mitochondria [mito–red fluorescent protein (RFP)] or TrkA (TrkA-mCherry). At 10 to 14 days in vitro, cargo motility (moving cargoes/total cargoes) and velocity (μm/s) were assessed before and after treatment with Aβ1-42 oligomers (2 μM) (12). Aβ rapidly inhibited axonal motility of mitochondria and TrkA in wild-type neurons. The effect was stronger on anterograde (mitochondria, P < 0.001; TrkA, P < 0.001) than retrograde (mitochondria, P < 0.01; TrkA, nonsignificant) transport. Complete or partial tau reduction prevented these defects without affecting axonal transport at baseline (Fig. 1 and movies S1 to S6). Moving cargoes had similar velocities in all Tau genotypes at baseline and after Aβ treatment.

Fig. 1

(A) Anterograde axonal movement of a single mitochondrion (yellow triangles) is shown in successive 5-s image frames. (B) The microtubule-depolymerizing agent nocodazole (10 μg/ml) and oligomeric Aβ1-42 (2 μM) inhibited mitochondrial movements in Tau+/+ axons within 60 min. Aβ with a scrambled amino acid sequence (Aβ1-42-Scrambled, 2 μM) had no effect. n = 7 to 24 axons per condition. **P < 0.01, ***P < 0.001 versus corresponding baseline (paired t tests with Bonferroni correction). (C) Aβ1-42 inhibited mitochondrial movements in Tau+/+ axons within 20 min. n = 7 to 8 axons for each data point. **P < 0.01 versus corresponding baseline (paired t tests, Bonferroni). (D to F) Aβ1-42 inhibited mitochondrial [(D) and (E)] and TrkA (F) motility in Tau+/+ axons within 60 min but not in Tau+/– or Tau–/– axons. n = 24 to 45 axons for each genotype and condition. **P < 0.01, ***P < 0.001 versus corresponding baseline (paired t tests, Bonferroni). The percent reduction in anterograde transport in the presence of Aβ was greater in Tau+/+ axons than in Tau+/– or Tau–/– axons [(D) and (F)]. #P < 0.05, ##P < 0.01 (Kruskal-Wallis analysis of variance, Dunn). Error bars are SEM. See also table S1.

Tau reduction did not enhance axonal transport under physiological conditions. However, tau levels were more critical for axonal transport in the presence of Aβ. Aβ oligomers impair axonal motility of cargoes through complex mechanisms involving N-methyl-d-aspartate receptor signaling (3), activation of glycogen synthase kinase 3β (3, 4) and casein kinase 2 (5), and actin polymerization (6). Why Aβ requires tau to impair axonal transport is uncertain; tau might interact directly or indirectly with any of these pathways or enhance the effects independently by competing with motor proteins for microtubule access (7). Although most concentrated in axons, tau may have Aβ-enabling activities also in dendrites (2).

Tau ablation induces axonal spheroids in Tg2576 APP transgenic mice (13), but the functional importance is unknown. Tau reduction prevents Aβ-induced neuronal and behavioral deficits in hAPPJ20 mice (1) and APP23 mice (2). Protection against Aβ-induced defects in axonal transport is one of several possible mechanisms for this rescue.

Although tau did not affect axonal transport under baseline untreated conditions in vitro (this study) or in vivo (9), it may still be important under physiologic conditions. For example, local tau gradients may promote cargo detachment at strategic points (7, 13). Tau may also regulate cellular transport of its binding partners (2), and tau reduction might have affected axonal transport of cargoes we did not assess. Partial tau reduction may strike a balance between therapeutic safety and efficacy because it prevented Aβ-induced axonal transport defects as well as aberrant neuronal activity (1), cognitive deficits (1), and premature mortality (1, 2) in hAPP mice. In addition to tau reduction strategies, components of the axonal transport machinery and of Aβ- or tau-related signaling cascades are potential therapeutic targets warranting further investigation.

Supporting Online Material

www.sciencemag.org/cgi/content/full/science.1194653/DC1

Materials and Methods

Table S1

Movies S1 to S6

References and Notes

  1. Materials and methods are available as supporting material on Science Online.
  2. We thank A. Chung, K. Ho, E. LaDow, H. Solanoy, X. Wang, and G. Yu for excellent technical support and advice; Y. Huang for helpful discussions on mitochondrial transport; Y. Yoon for the mito-RFP plasmid; H. M. Brown for the TrkA-mCherry plasmid; M. Vitek and H. Dawson for tau knockout mice; A. Holloway for statistical support; S. Ordway for editorial review; J. Carroll for graphics support; and M. Dela Cruz for administrative assistance. L.M. has received research funding from Elan Pharmaceuticals for other projects and serves on the Scientific Advisory Boards of AgeneBio, iPierian, and Probiodrug. This work was supported by NIH grants AG011385 and NS041787 (L.M.) and NS057906 (B.C.) and the McBean Family Foundation (K.A.V.). The animal care facility was partly supported by an NIH Extramural Research Facilities Improvement Program Project (C06 RR018928).

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