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BID, BIM, and PUMA Are Essential for Activation of the BAX- and BAK-Dependent Cell Death Program

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Science  03 Dec 2010:
Vol. 330, Issue 6009, pp. 1390-1393
DOI: 10.1126/science.1190217

Deadly Trio

The proteins BAX and BAK act as a key decision point, regulating apoptosis by controlling the permeability of the mitochondrial outer membrane. Evidence has been presented for two mechanisms of activation of BAX and BAK: an indirect mechanism where proapoptotic proteins neutralize the antiapoptotic effects of the protein BCL-2 and its relatives; or direct activation of BAX and BAK by BIM, BID, or PUMA. Analysis of the situation in vivo is complicated by the overlapping function of BIM, BID, and PUMA. Ren et al. (p. 1390; see the Perspective by Martin) thus analyzed triple-knockout mice lacking BIM, BID, and PUMA. Apoptosis during mouse development required a direct effect of one of these proteins to activate BAX or BAK, thereby promoting cell death.

Abstract

Although the proteins BAX and BAK are required for initiation of apoptosis at the mitochondria, how BAX and BAK are activated remains unsettled. We provide in vivo evidence demonstrating an essential role of the proteins BID, BIM, and PUMA in activating BAX and BAK. Bid, Bim, and Puma triple-knockout mice showed the same developmental defects that are associated with deficiency of Bax and Bak, including persistent interdigital webs and imperforate vaginas. Genetic deletion of Bid, Bim, and Puma prevented the homo-oligomerization of BAX and BAK, and thereby cytochrome c–mediated activation of caspases in response to diverse death signals in neurons and T lymphocytes, despite the presence of other BH3-only molecules. Thus, many forms of apoptosis require direct activation of BAX and BAK at the mitochondria by a member of the BID, BIM, or PUMA family of proteins.

Mitochondria have key roles in mammalian apoptosis, a highly regulated genetic program of cell suicide (13). Multiple apoptotic signals release cytochrome c from the mitochondria to activate the Apaf-1 protein, which activates caspases. The BCL-2 family of proteins integrates developmental and environmental cues to dictate the survival or death decision of cells by regulating the integrity of the mitochondrial outer membrane (MOM) (1, 4, 5). The multidomain proapoptotic proteins BAX and BAK mediate permeabilization of the MOM, whereas antiapoptotic BCL-2, BCL-XL, and MCL-1 proteins prevent cytochrome c efflux triggered by apoptotic stimuli. The third BCL-2 subfamily of proteins, the BH3-only molecules (BH3s) (that is, family members with only one BCL-2-homology domain), constitutes the largest BCL-2 subfamily with more than 10 members that promote apoptosis by either activating BAX and BAK directly or inactivating BCL-2, BCL-XL, or MCL-1 (612). When apoptosis is initiated, BAK and BAX undergo conformational changes to form homo-oligomers that mediate cytochrome c efflux (69). Although BAX and BAK control the mitochondrial gateway to apoptosis, how BAX and BAK are activated, whether BAX and BAK are activated directly or indirectly by BH3s, and the identity of the core repertoire of activators of BAX and BAK in various tissues remain unsettled. Two non–mutually exclusive models have been proposed (5, 13). The direct activation model states that the “activator” subgroup of BH3s, including truncated BID (tBID) and BIM, can directly induce the conformational changes of BAX and BAK (612, 1416). The indirect model proposes that activation of BAX and BAK occurs by default as long as all the antiapoptotic BCL-2 proteins are neutralized by BH3s, based on the observation that BAX- or BAK-dependent apoptosis proceeds in the absence of BID and BIM (17). However, PUMA appears also to function as a direct activator of BAX and BAK. In vitro translated PUMA protein, but not the BH3 domain peptide of PUMA, can directly activate BAX- and BAK-dependent permeabilization of the MOM (11, 12, 1820). The transmembrane domain was important for PUMA to induce cytochrome c efflux (fig. S1), which may explain why BH3 peptides are less active (11, 19). Thus, we studied Bid−/−Bim−/−Puma−/− triple-knockout (TKO) mice to clarify how BAX and BAK are activated and whether BID, BIM, and PUMA represent the core repertoire of activators of BAX and BAK that function downstream of other BH3s.

Although the embryonic lethality caused by triple deficiency of Bid, Bim, and Puma appeared to be less severe than that in mice lacking Bax and Bak (table S1), the viable Bid−/−Bim−/−Puma−/− mice displayed developmental defects very similar to those of Bax−/−Bak−/− animals, including persistent interdigital webs of skin on their feet and imperforate vaginas (21) (Fig. 1, A and B, and table S2). To determine whether deficiency of Bid, Bim, and Puma completely blocks the intrinsic apoptotic death as deficiency of Bax and Bak does, we examined ionizing radiation (IR)–induced apoptosis in cerebellar granule neurons (CGN). Because most of the Bax−/−Bak−/− mice die in embryogenesis, we used the Cre-LoxP conditional knockout strategy to delete Bax in neurons through a Nestin-Cre construct that is expressed in neuronal and glial cell precursors (22). No activated caspase-3 was detected in Baxf/–Bak−/−Nestin-Cre+ neurons (Fig. 1D). Puma-deficient neurons were less sensitive to IR than Bax-deficient neurons (Fig. 1E and fig. S2). Although deficiency of Puma greatly reduced the activation of caspase-3, deletion of Bid, Bim, and Puma was required to completely block caspase-mediated apoptosis (Fig. 1, E to H). Similar findings were also observed in IR-induced apoptosis in dentate gyrus. BAD and BMF proteins were detected in CGN (fig. S3), suggesting that BAD and BMF were unable to activate BAX- or BAK-dependent apoptosis in the absence of Bid, Bim, and Puma. These data indicate that activation of BAX and BAK in response to IR is fully dependent on BID, BIM, and PUMA.

Fig. 1

Triple deficiency of Bid, Bim, and Puma phenocopies double deficiency of Bax and Bak. (A) Bid−/−Bim−/−Puma−/− TKO mice display persistence of interdigital webs. Ventral views of paws from WT, Bid−/−Bim−/−, and Bid−/−Bim−/−Puma−/− mice. (B) Bid−/−Bim−/−Puma−/− TKO mice fail to develop external vaginal introituses. Photographs of vaginal openings from WT, Bid−/−Bim−/−, and Bid−/−Bim−/−Puma−/− mice. Arrows point to external vaginal region. (C to G) Immunohistochemistry for cleaved caspase-3 from cerebella of postnatal day 5 (P5) mice of the indicated genotypes that were irradiated with 14 Gy γ-irradiation. (C) WT, 18 hours after IR. (D) Baxf/-Bak−/−Nestin-Cre+, 30 hours after IR. (E) Puma−/−, 30 hours after IR. (F) Bim−/−Puma−/−, 30 hours after IR. (G) Bid−/−Bim−/−Puma−/−, 30 hours after IR. Arrows denote the external granular layer of cerebellum. Data shown are representative images of two to three independent experiments. (H) Analyses of a whole sagittal section of cerebellum at the same level from experiments shown in (C) to (G) summarize the numbers of neurons at the external granular layer that were stained positive for cleaved caspase-3. Data shown are the average of two independent experiments (n = 2).

To exclude the possibility that BID, BIM, and PUMA are required to activate BAX or BAK only in response to genotoxic stress, we investigated potassium-deprivation–induced apoptosis of cultured CGN. CGN from early postnatal mice can be cultured in medium containing a high concentration of potassium (25 mM) that provides depolarization-mediated survival. Deprivation of potassium (5 mM) induces BAX-dependent apoptosis that requires de novo protein synthesis (23). Consistent with the previous reports demonstrating the accumulation of BIM and PUMA proteins in potassium-deprived CGN (24, 25), deficiency of either Bim or Puma provided transient protection, whereas deficiency of both Bim and Puma conferred long-term protection against potassium-deprivation–induced apoptosis (Fig. 2 and figs. S4 and S5). Deletion of Bid, Bim, and Puma completely blocked apoptosis up to 3 days (Fig. 2 and figs. S4 and S5). These data indicate that BAX is not activated in the absence of Bid, Bim, and Puma, even though BAD and BMF are present (fig. S3) and the abundance of HRK (also called DP5) is increased in these neurons (26).

Fig. 2

Bid−/−Bim−/−Puma−/− TKO cerebellar granule neurons are completely resistant to potassium-deprivation–induced apoptosis. Cerebellar granule neurons from WT, Bim−/−, Puma−/−, Bim−/−Puma−/−, or Bid−/−Bim−/−Puma−/− mice were cultured in high-K+ (K25 + S) for 7 days and then transferred to low-K+ medium (K5 + S) to induce apoptosis. Viability was determined at the indicated times using propidium iodide (PI) staining. Data are the mean percentage of PI-positive neurons ± SD from three independent experiments. **, P < 0.01; ***, P < 0.001.

One of the major phenotypes of Bax−/−Bak−/− mice is the accumulation of lymphoid and myeloid cells as a consequence of defective apoptosis (21). Bid−/−Bim−/−Puma−/− animals also exhibited enlarged thymi, splenomegaly, and lymphadenopathy (fig. S6). The role of PUMA as a direct activator of BAX and BAK is debated in part because of reports showing that knockdown of Puma does not provide further inhibition of apoptosis in the absence of Bid and Bim (17) and because exogenous PUMA fails to induce BAX- and BAK-dependent mitochondrial permeabilization in the absence of Bid and Bim (27). To address whether endogenous PUMA can activate BAX- and BAK-dependent mitochondrial apoptosis independently of BID and BIM, we compared the apoptotic phenotypes of Bid−/−Bim−/− double-knockout (DKO) mice to Bid−/−Bim−/−Puma−/− TKO mice. The TKO mice had more pronounced accumulation of hematopoietic cells in thymus, spleen, lymph nodes, and blood than DKO mice (figs. S6 to S8). Moreover, Bid−/−Bim−/−Puma−/− TKO T cells were more resistant than Bid−/−Bim−/− DKO cells to diverse intrinsic apoptotic signals, including cytokine deprivation, DNA damage (IR or etoposide), glucocorticoids (dexamethasone), or endoplasmic reticulum (ER) stress (tunicamycin) (Fig. 3 and fig. S9). Puma-deficient cells were less resistant to apoptosis than TKO cells (fig. S10). Indeed, deficiency of Bid, Bim, and Puma appears to provide more protection against intrinsic apoptotic death than deficiency of Apaf-1 or Capase-9 (28). By analogy to Bax−/−Bak−/− T cells (21), Bid−/−Bim−/−Puma−/− T cells were still sensitive to Fas-induced apoptosis because the BCL-2 family does not regulate extrinsic apoptotic death in T cells (Fig. 3E). The proapoptotic activity of BID is activated upon its cleavage by proteases such as caspase-8 or -2 (1, 4, 5). Cleavage of BID was detected in thymocytes in response to DNA damage or ER stress (fig. S11).

Fig. 3

Bid−/−Bim−/−Puma−/− TKO T cells are resistant to diverse apoptotic stimuli. (A to E) CD4+ T cells purified from the spleens of WT (n = 3), Bid−/−Bim−/− (n = 3), or Bid−/−Bim−/−Puma−/− (n = 3) mice at 8 to 10 weeks of age were cultured under the following conditions: in the absence of cytokine (A), after exposure to 2.5 Gy γ-irradiation (B), in the presence of etoposide (C), in the presence of dexamethasone (D), or in the presence of agonistic antibody to Fas (E). Cell death was quantified by annexin-V staining at the indicated times. (F to I) Thymocytes from WT (n = 3), Bid−/−Bim−/− (n = 3), or Bid−/−Bim−/−Puma−/− (n = 3) mice at 6 to 8 weeks of age were cultured under the following conditions: in the absence of cytokine (F), after exposure to 2.5 Gy γ-irradiation (G), in the presence of etoposide (H), or in the presence of tunicamycin (I). Cell death was quantified by annexin-V staining at the indicated times. Data are the mean percentage of annexin-V–positive cells ± SD from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

To investigate whether activation of BAX and BAK was blocked in the absence of Bid, Bim, and Puma, gel filtration was performed to assess homo-oligomerization of BAX or BAK. In both wild-type (WT) and Bid−/−Bim−/−Puma−/− TKO cells, BAX was eluted as a 20-kD monomer from untreated cells (Fig. 4A). After IR or treatment of cells with tunicamycin, BAX formed higher-ordered oligomers in WT but not TKO cells (Fig. 4A). Similarly, higher-ordered oligomers of BAK were only detected in WT cells in response to genotoxic or ER stress (Fig. 4B). Consistent with the lack of homo-oligomerization of BAX or BAK detected in TKO cells, cytochrome c translocation and caspase activation were not observed in these cells when exposed to diverse intrinsic apoptotic signals (Fig. 4, C and D, and figs. S12 and S13). Although the majority of WT cells lost cytochrome c immunostaining within 20 hours after IR, in TKO cells, cytochrome c remained in the mitochondria, exhibiting a punctate staining pattern (Fig. 4C). Caspase activation determined by the cleavage of poly (ADP-ribose) polymerase (PARP) and caspase-3 was not observed in TKO cells treated with IR, dexamethasone, or tunicamycin (Fig. 4D). Notably, tBID, BIM, and PUMA were potent death agonists in both WT and Bid−/−Bim−/−Puma−/− TKO cells (Fig. 4E and fig. S14).

Fig. 4

BID, BIM, and PUMA are required to activate BAX- and BAK-dependent mitochondrial apoptosis. (A and B) Triple deficiency of Bid, Bim, and Puma prevents activation of BAX and BAK. Thymocytes from WT or Bid−/−Bim−/−Puma−/− mice were untreated, irradiated with 5 Gy γ-irradiation (IR), or treated with tunicamycin (TC). Protein lysates were harvested at 7 hours after IR or 20 hours after TC treatment and subjected to Superdex 200 (HR10/30) gel-filtration chromatography. Fractions were analyzed by Western blot using antibodies to BAX (A) or BAK (B). (C) Triple deficiency of Bid, Bim, and Puma prevents the translocation of cytochrome c. Fluorescence microscopy of WT or Bid−/−Bim−/−Puma−/− thymocytes 20 hours after exposure to 2.5 Gy γ-irradiation. Red represents cytochrome c immunostaining, and blue is Hoechst staining. White asterisks indicate apoptotic cells that have lost cytochrome c. (D) Triple deficiency of Bid, Bim, and Puma prevents the activation of caspases. Thymocytes from WT or Bid−/−Bim−/−Puma−/− mice were untreated, treated with TC or dexamethasone (Dex), or irradiated with 5 Gy γ-irradiation (IR). After 12 hours, protein lysates were harvested and assessed by Western blot using antibodies specific for PARP, cleaved PARP, cleaved caspase-3, or actin. Asterisk denotes a cross-reactive protein. (E) Primary mouse embryonic fibroblasts isolated from WT or Bid−/−Bim−/−Puma−/− TKO mice were infected with retroviruses expressing the indicated genes. Cell death was quantified by annexin-V staining at 24 hours. Data are the mean percentage of annexin-V–positive cells ± SD from three independent experiments. *, P < 0.05.

The lack of strong and stable interaction between BH3s and BAX/BAK was thought to support the indirect activation model for BAX and BAK. However, dynamic interactions occur between BAX and activator BH3s, including tBID, BIM, and PUMA (18), which helps explain the previous difficulty in detecting these interactions. Here, we demonstrate an essential role of BID, BIM, and PUMA in activating BAX and BAK and for some apoptotic events during development. Our genetic study indicates that BAD is unable to induce apoptosis in the absence of BID, BIM, and PUMA, which is further supported by the observation that the BAD mimetic, ABT-737 (29), failed to kill Bid−/−Bim−/−Puma−/− TKO cells (fig. S15). These data suggest that the profound block of apoptosis conferred by the triple deficiency of Bid, Bim, and Puma is not simply caused by altering the ratio between antiapoptotic and proapoptotic BCl-2 proteins. BH3s not only induce BAX- and BAK-dependent release of cytochrome c to activate caspases but also initiate caspase-independent mitochondrial dysfunction (9). Accordingly, persistent interdigital webs and accumulation of hematopoietic cells were observed in Bax−/−Bak−/− DKO (21) or Bid−/−Bim−/−Puma−/− TKO mice, but not in Apaf-1–deficient mice (28, 30). Overall, our study reveals an essential axis of activator BH3s and BAX and BAK in activating the mitochondrial death program, which offers common ground for therapeutic interventions.

Supporting Online Material

www.sciencemag.org/cgi/content/full/330/6009/1390/DC1

Materials and Methods

Figs. S1 to S15

Tables S1 and S2

References

References and Notes

  1. We apologize to all the investigators whose research could not be appropriately cited owing to space limitation. We thank T. D. Westergard and H.-F. Chen for technical assistance. This work was supported by grants to E.H.-Y.C. from NIH (R01CA125562) and the Searle Scholars Program, and to G.P.Z. from NIH (R01GM083159 and P30CA21765)
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