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Sex Determination in the Social Amoeba Dictyostelium discoideum

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Science  10 Dec 2010:
Vol. 330, Issue 6010, pp. 1533-1536
DOI: 10.1126/science.1197423

Abstract

The genetics of sex determination remain mysterious in many organisms, including some that are otherwise well studied. Here we report the discovery and analysis of the mating-type locus of the model organism Dictyostelium discoideum. Three forms of a single genetic locus specify this species' three mating types: two versions of the locus are entirely different in sequence, and the third resembles a composite of the other two. Single, unrelated genes are sufficient to determine two of the mating types, whereas homologs of both these genes are required in the composite type. The key genes encode polypeptides that possess no recognizable similarity to established protein families. Sex determination in the social amoebae thus appears to use regulators that are unrelated to any others currently known.

Most eukaryotes are sexual, but little is known in molecular detail about sex across most branches of the eukaryotic tree. One aspect, the genetic basis of sex determination, is well understood in several animal, fungal, and plant lineages (15), but across the protozoan kingdoms we know little, and nothing in comparable detail. The social amoebae are members of the Amoebozoa and have an unusual sexual cycle that leads to the formation of dormant walled macrocysts (6) (Fig. 1, A and B). To produce a macrocyst, a pair of haploid amoebae of different sexes fuse (7) to form a diploid zygote, which then attracts surrounding haploid cells (8). These help to lay down external layers of cellulose around the developing mass of cells before being cannibalized by the zygote (9). After a period of dormancy, the cyst germinates, releasing haploid progeny that arise most likely after meiosis and multiple mitoses (10). The population genetics of wild isolates indicate that mating and recombination are probably frequent in the wild (11).

Fig. 1

The sexual cycle of D. discoideum. (A) Amoebae of different sexes (top) first fuse, and several hours later their two nuclei also fuse, resulting in a diploid zygote (top right). This then secretes cyclic adenosine monophosphate to attract surrounding haploid cells (bottom right), which are ingested by the zygote (bottom); eventually a dormant macrocyst is formed, retaining partly digested haploids within vacuoles (left). Ultimately the macrocyst germinates, releasing tens or hundreds of progeny, all descending from the zygote. (B) Early macrocysts (precysts) of strain AC4, formed in shaken suspension approximately 24 hours after removal of food bacteria. Here, two developing macrocysts are contained within the same outer wall. In each cyst, inner walls envelop hundreds of haploid amoebae and the zygote, which is visible here as the darker cell mass at the center of each cyst, and which slowly eats its way out to the limiting wall. Within the zygotes, the structures of ingested amoebae are still clear. Scale bar, 50 μm.

The most-studied species of social amoeba, Dictyostelium discoideum, is notable for having three sexes [hereafter called mating types I, II, and III [supporting online material (SOM) text S1], as well as uncommon self-fertile homothallic strains (1214). Each of the three sexes can pair with each of the other two but not with itself, giving three possible classes of zygote: type I/type II, type I/type III, and type II/type III. Although several genes are known to be involved during the sexual cycle (15), the determinant of mating type has proved elusive. Genetic analysis suggested that mating type is stable and is determined by a single locus with two or more alleles (10, 14, 16). We argued that it might be possible to identify this postulated locus by searching for genes that are present in any member of one mating type but absent or highly diverged in any member of another. For this purpose, we performed comparative genomic hybridizations using DNA microarrays composed of probes for around 8500 of the 10,500 predicted genes in the sequenced type I D. discoideum genome (17).

We analyzed 10 strains derived from independent wild isolates (table S1) using this microarray (18) and found a single candidate gene that follows the pattern expected of a sex-determining sequence: It is present in all type I genomes but absent from all of the type II strains (Fig. 2, fig. S1, and table S2). This open reading frame (ORF) is situated on chromosome 5 (SOM text S2) and is very short, encoding a 107–amino acid polypeptide that contains no significant homology to previously studied proteins. No motifs or potential domains suggest a function, but it is relatively highly charged and so is most likely a soluble intracellular protein. We confirmed that the ORF is present in all the other type I strains used in the microarray study (100% identical in amino acid sequence in all cases; table S3), and sequenced the entire locus from another type I strain, WS205, to confirm that no other obvious coding sequences are present.

Fig. 2

Identification of a candidate mating-type locus. Our search assumed that the mating-type locus would differ substantially in sequence between mating types, so using a microarray designed from type I sequence, we sought genes that were present in all examples of this mating type and absent from or highly diverged in all examples of type II. Out of more than 8500 genes covered on the array, only one, matA (alongside the red star), behaved in this way when 10 independent wild isolates were compared with our laboratory strain Ax2 in DNA-to-DNA comparisons (strain names are shown at the bottom). The heat map shows only the set of genes giving a log ratio below –2 in at least one of the wild strains as compared to Ax2 (full data are presented in table S2). Each row in the plot represents a gene, each column a strain. Blocks are colored according to log(2)ratio, from blue (negative: decreased copy number or sequence divergence in the test strain) through white (zero: no difference) to red (positive: increased copy number in the test strain). Several other sequences apart from matA were absent or diverged in different isolates, but none of these correlates with mating type; it should be noted that NC4 is the ultimate parent of Ax2, accounting for the similarity between them.

To test whether the gene is involved in the sexual cycle, we deleted it from our standard type I strain (19). The resulting mutant, in contrast to its parent, is unable to form macrocysts when paired with a type II strain (Fig. 3). When the coding sequence is reintroduced under the control of a constitutive promoter, mating competence is restored (Fig. 3D and table S4), showing that this gene, which we call matA, is necessary for correct type I mating behavior, and supporting the proposition that it is involved in sex determination.

Fig. 3

Re-engineering the mating behavior of a type I strain. The type I strain Ax2, which can mate with type II strain V12M2 (A), was first modified by the deletion of matA. The resultant strain is unable to mate with V12M2 (B) or any other strain. The introduction of matC with its own regulatory sequences into this null mutant gives a strain that is able to mate with its ultimate parent Ax2 (C). Structures were imaged 7 days after mixing, by means of differential interference contrast microscopy. Scale bar, 50 μm. (D) Macrocysts formed in various crosses were counted 8 days after mixture of strains. Eleven strains were crossed with a type I strain, Ax2 (left), and with a type II strain, V12M2 (middle): WT-I is the parental Ax2 strain and “null” is the matA deletion strain in this background. The next nine strains are the null plus one or more mat genes controlled by a constitutive promoter; each is designated by the gene's letter. These strains, apart from the matA-expressing strain, and also the type II strain V12M2 (WT-II), were also crossed with the type III strain WS2162 (right). The mean number of macrocysts plus and minus the standard error from three independent crosses are plotted.

The two genes flanking matA in the reference type I sequence are present in all strains and do not vary in amino acid sequence between mating types (fig. S2). We could therefore amplify the entire type II version of the locus by polymerase chain reaction from the NC66.2 isolate. The type II version is larger and contains three genes (Fig. 4A), one of which, matB, is homologous to matA but considerably diverged at approximately 60% identity in amino acid sequence (fig. S3 and SOM text S1). A second small gene, matC, encodes a 208–amino acid hydrophilic polypeptide that again has no similarity to other known proteins (fig. S4). The third gene, matD, is larger, spliced, and encodes a 799–amino acid preprotein that contains a predicted signal peptide and potential glycosylphosphatidylinositol-anchor attachment site (fig. S5). No homology is shared between these three genes. All of the other type II strains tested contain the same three genes, with >98% sequence identity (table S4), and the complete type II locus from the Japanese type II isolate NYA64 was sequenced, again confirming that no additional coding sequences are present.

Fig. 4

The structure and logic of the D. discoideum mat locus. (A) Type I strains are characterized by a single protein-coding gene, matA (blue, A; dictyBase identification no. DDB_G0289165), which is homologous to matB (also blue, B), one of the three genes present in the type II version of the locus. The two other genes making up the type II locus, matC (yellow, C) and matD (green, D), are homologous to the two genes that occupy the type III version, matS and matT (yellow and green according to homology, S and T; gene nomenclature is treated further in the SOM text). The locus lies on chromosome 5 between the genes DDB_G0289171 and DDB_G0289163, which do not vary according to mating type and are shown here in gray. (B) Mating compatibility requires the presence of a matA-class gene (blue triangles) and a matS-class gene (yellow circles) in the two gametes. Type II cells contain a gene of each class, allowing mating with both types I and III. The nature of the interactions between genes remains unknown, as does the molecular explanation of how the matB and matC pair are incompatible.

To prove that this locus is responsible for sex determination, we attempted to switch the mating type of our type I laboratory strain. The matA gene was first deleted and the resulting mutant then transformed with a construct bearing the entire type II version of the locus. The resulting strain then behaved as type II (SOM text S3), forming macrocysts when paired with type I cells but not with type II (table S5). This reversal of mating orientation demonstrates that this locus is sufficient to specify mating types I and II and that we have identified the Dictyostelium mating-type locus.

Next we characterized the locus in the type III strain WS2162. This version contains two genes homologous to matC and matD but divergent in sequence, and which we name matS and matT, respectively (Fig. 4A and figs. S4 and S5). No sequence related to matA or matB is present. The same pattern is followed in another type III isolate, WS112B (table S6). Each mating type thus possesses a different version of the mating-type locus, and there is no overlap between the type I and type III versions. The type II version resembles a composite of the other two, containing homologs of the genes from the type I and type III versions. Two homothallic isolates resemble type III, containing genes related to matS and matT but no matA/matB homolog (table S6 and SOM text S4).

Because mating type I is determined by a single gene, we next asked whether any of the genes at the type II and type III versions of the locus could act as master regulators of sex determination, with others perhaps having subsidiary roles. To do this, we expressed individual genes, either alone or in combination, in the matA null strain, which is unable to mate with any strain. The type II locus was analyzed in most detail. Expressing matB alone from a constitutive promoter is sufficient to produce a strain able to mate with a type III strain, but not with types I and II (Fig. 3D and table S5). Thus, the homologous matA and matB genes appear to have similar but not identical functions: The former specifies mating competence toward type II and type III, whereas the latter is only effective toward type III, and matB only partially accounts for the type II phenotype.

Expressing matC alone in the same null background allows mating with type I cells but not type III or type II cells (Fig. 3D and table S5). We obtained the same result when we replaced the knocked-out version of the matA locus with a truncation of the NC66.2 mat locus containing only matC and its native regulatory sequences; again, this strain, which now contained only matC in place at the mating locus on chromosome 5, mated efficiently with type I cells but not at all with type III or type II cells (Fig. 3C and table S5). When the knocked-out version is replaced with the genomic region bearing both matB and matC, under the control of their own promoters, the resulting strain can form macrocysts with both types I and III (table S5). Similarly, overexpression of both matB and matC in the same strain recapitulates normal type II behavior as adjudged by this assay (Fig. 3D and table S5). These strains are not self-fertile (table S5). Therefore, two unrelated mat genes together specify the type II mating type.

The final gene contained in the type II version of the locus, matD, is not required for mating-type determination. When expressed alone in the mating-null cells from a constitutive promoter, it does not allow mating with any of the mating types, and it does not affect the qualitative behavior of matB or matC when expressed in combination with them, although there is some evidence of quantitative effects. Yields of macrocysts are higher when matD is present in some crosses (table S5); this is consistent with a possible role in promoting gamete fusion.

Turning to the type III version, the presence of a matC homolog suggested that this gene, matS, might specify this third mating type. As expected, expressing matS in the matA null background gives a strain that mates with type I and type II but not with type III (Fig. 3D and table S5). Furthermore, crosses between strains bearing just the master control genes give the expected results: Null cells expressing just matS can mate with cells expressing just matB but not with cells expressing just matC (table S5). Finally, cells separately expressing matB and matC do not mate with each other (table S5). We have not analyzed matT separately, but like matD it does not appear to be necessary for sex determination.

These results suggest a simple underlying picture: type I and type III mating behavior can be specified by a single gene in each case: matA specifies type I and matS specifies type III. Type II is a composite in which homologs of matA and matS (matB and matC, respectively) allow it to mate with the other two mating types but, for reasons that remain unclear, not with itself (Fig. 4B).

The molecular function of these genes remains to be addressed. No clear homologs are present in species outside of the dictyostelids, but D. purpureum possesses two adjacent genes that are homologous to matS and matT (figs. S4 and S5). A clear matD/matT homolog is also evident in the more distantly related dictyostelid Acytostelium subglobusum (fig. S5). Adjacent to this gene is a small ORF very weakly similar to matC/matS, a possible matS ortholog in this species (fig. S6). MatD is distantly related to the Hap2-GCS1 family of gamete fusion proteins (20), supporting the idea that MatD and MatT are involved during fusion, although another D. discoideum gene, hapA, encodes a protein much more closely related to the canonical Hap2 group and is enriched in cells competent for mating (21).

The organization of the Dictyostelium mating-type locus does not closely resemble previously studied sex-determining regions (SOM text S5), although like them the mat locus must ultimately control a transcriptional cascade (21). Whether the key genes directly regulate transcription or do so through downstream targets remains to be determined. One further role could be in controlling mitochondrial inheritance, which is uniparental in related Amoebozoans (22, 23).

We have identified the sex-determining locus from D. discoideum, a model organism and the best-studied member of the Amoebozoa. The master regulators of sex determination at this locus encode homologs of two small, apparently soluble proteins, which are unrelated to previously studied proteins. The genetic logic of the system allows one to speculate that one sex, mating type II, may have arisen after a fusion of the versions of the locus of the other two sexes. Understanding the mating-type locus may help to overcome longstanding difficulties in making use of sexual genetics in Dictyostelium (24).

Supporting Online Material

www.sciencemag.org/cgi/content/full/330/6010/1533/DC1

Materials and Methods

SOM Text S1 to S5

Figs. S1 to S6

Tables S1 to S6

References

References and Notes

  1. Materials and methods are available as supporting material on Science Online.
  2. This work was supported by Wellcome Trust grant number 06724 and core funding from the Medical Research Council. We thank the Dicty Stock Center (www.dictybase.org/StockCenter/StockCenter.html), D. Francis, H. Hagiwara, and P. Schaap for providing strains; T. Feltwell, N. Nikolaidou-Katsaridou, and K. Jagels for technical assistance; and M. Fookes, C. Carret, K. Brooks, C. Pears, N. Barry, and the members and associate members of the Dictyostelium group at the Laboratory of Molecular Biology for valuable advice and discussions. A.I. is now at Fios Genomics, ETTC, King’s Buildings, Edinburgh EH9 3JL, UK. We are grateful to the U.S. Department of Energy Joint Genome Institute (www.jgi.doe.gov/) and co-workers, and to the Actyostelium Genome Consortium for making the Dictyostelium purpureum and Acytostelium subglobosum genome data available before publication. Microarray data are available under accession no. E-TABM-394 and the array design under A-SGRP-3 at www.ebi.ac.uk/arrayexpress/, and DNA sequences have been deposited in EMBL-Bank under accessions nos. FN543120 to FN543124, FN994780, FR666792, and FR666793.
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