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The Legionella Effector RavZ Inhibits Host Autophagy Through Irreversible Atg8 Deconjugation

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Science  23 Nov 2012:
Vol. 338, Issue 6110, pp. 1072-1076
DOI: 10.1126/science.1227026

Abstract

Eukaryotic cells can use the autophagy pathway to defend against microbes that gain access to the cytosol or reside in pathogen-modified vacuoles. It remains unclear if pathogens have evolved specific mechanisms to manipulate autophagy. Here, we found that the intracellular pathogen Legionella pneumophila could interfere with autophagy by using the bacterial effector protein RavZ to directly uncouple Atg8 proteins attached to phosphatidylethanolamine on autophagosome membranes. RavZ hydrolyzed the amide bond between the carboxyl-terminal glycine residue and an adjacent aromatic residue in Atg8 proteins, producing an Atg8 protein that could not be reconjugated by Atg7 and Atg3. Thus, intracellular pathogens can inhibit autophagy by irreversibly inactivating Atg8 proteins during infection.

Legionella pneumophila is an intracellular pathogen that manipulates evolutionarily conserved membrane transport pathways to create a specialized vacuole that supports bacterial replication in host cells (1). Legionella requires a type IV secretion system called Dot/Icm to replicate intracellularly (2, 3). Effector proteins that modulate membrane transport are translocated into host cells by the Dot/Icm system (1, 4).

The autophagy pathway is used by eukaryotic cells to sequester cytosolic proteins and organelles into a membrane-bound compartment called an autophagosome (AP), which fuses with lysosomes to promote cargo degradation (5). Autophagy can be used by plant and animal cells to target intracellular pathogens for degradation in lysosomes (6, 7). An essential step in the autophagy pathway is the coupling of an Atg8 homolog to the lipid phosphatidylethanolamine (PE) on early AP structures (8). The most widely studied Atg8 protein in mammalian cells is the microtubule-associated protein light chain 3 (LC3) (9). Unconjugated LC3 (LC3-I) runs more slowly by SDS–polyacrylamide gel electrophoresis (SDS-PAGE) than the lipidated form of LC3 (LC3-II), which means that measuring LC3-II levels by immunoblot analysis provides an indication of autophagy activity in a cell (10).

Here, we measured LC3-II levels during infection of host cells by Legionella to determine if this pathogen modulates autophagy (see supplementary materials and methods). LC3-II levels were reduced in human embryonic kidney (HEK) 293 cells infected with a virulent strain of Legionella pneumophila Philadelphia-1 when compared with uninfected cells (Fig. 1A). The block in LC3-II generation was more apparent when degradation of LC3-II was prevented upon treatment of cells with bafilomycin A1 to neutralize lysosomal pH. LC3-II levels were not affected when cells were infected with an isogenic Legionella dotA mutant (11), which has a nonfunctional Dot/Icm system (Fig. 1A). Uninfected cells and cells infected with the dotA mutant contained punctate LC3-positive APs resulting from basal levels of autophagy, whereas punctate LC3-positive APs were absent in most cells infected with virulent Legionella (Fig. 1, B and C). Additionally, LC3-II levels were low in primary macrophages that had been injected by the Dot/Icm system (Fig. 1D and fig. S1), and LC3 puncta were absent in these infected macrophages (Fig. 1, E and F). Thus, Legionella has a Dot/Icm-dependent mechanism to inhibit the autophagy pathway.

Fig. 1

Legionella inhibits autophagy by a Dot/Icm-dependent mechanism. (A) Immunoblot analysis of LC3-I and LC3-II levels in uninfected cells (control) and cells infected for 2 hours with either wild-type (WT) Legionella or a dotA mutant. Treatment of cells with bafilomycin A1 is indicated. (B) Images show LC3 (green) distribution in HEK293 cells infected with the indicated Legionella strains (red) for 2 hours. Scale bars, 1 μm. (C) Percent of cells containing LC3-puncta calculated from three independent assays, where a total of 100 cells were scored in each assay. Data represent the average ± SD (error bars); *P < 0.0001 compared to the uninfected control. (D) Mouse bone marrow–derived macrophages were infected for 2 hours with a LegionellaflaA strain producing a BlaM-RalF fusion protein. Immunoblot analysis was used to detect LC3-I and LC3-II levels in injected and uninjected cells (fig. S1). (E) Representative images of bone marrow–derived macrophages from GFP-LC3 transgenic mice infected with the indicated Legionella strains (red) for 2 hours. Cells were stained using an antibody specific for GFP (green). Scale bars, 1 μm. (F) GFP-LC3 puncta staining for uninfected and infected mouse bone marrow–derived macrophages. The percent of cells containing GFP-LC3 puncta was calculated from three independent assays where a total of 100 cells were scored in each assay. Data represent the average ± SD; *P < 0.0001 compared to the uninfected control.

Isogenic Legionella strains with large chromosomal deletions were used to determine the genetic basis for autophagy inhibition (12). Autophagy inhibition was not observed after infection with a “pentuple” mutant with five large chromosomal deletions that eliminate 71 putative effectors (Fig. 2A). Autophagy inhibition was also lost in the ∆3 mutant, which is missing only 10 of the effectors deleted in the pentuple mutant (Fig. 2A). When these 10 effectors were produced individually as green fluorescent protein (GFP)–effector fusions in HEK293 cells, only cells producing the GFP-RavZ fusion protein displayed low LC3-II levels (Fig. 2B). GFP-RavZ also prevented the formation of LC3 puncta in transfected HEK293 cells (fig. S2) and interfered with autophagy-dependent degradation of the long-lived protein substrates (Fig. 2C). Thus, RavZ can block autophagy.

Fig. 2

The Legionella effector protein RavZ is necessary and sufficient for autophagy inhibition. (A) Immunoblot analysis of LC3-I and LC3-II levels in HEK293 cells infected for 2 hours with either WT Legionella, a dotA mutant, the pentuple mutant, and mutants with single chromosomal deletions (∆2ab, ∆3, ∆4a, ∆6a, and ∆7a). (B) Immunoblot analysis of LC3-I and LC3-II levels in HEK293 cells producing the indicated Legionella effector proteins fused to GFP. (C) Long-term protein degradation was measured in cells producing GFP alone, GFP-RavZ, and GFP-RavZC258A. No significant differences were observed for cells grown in complete medium (black bars). An increase in protein degradation resulting from starvation-induced autophagy was observed in cells producing GFP alone or GFP-RavZC258A, but cells producing GFP-RavZ displayed a significant defect in autophagy-dependent protein degradation. Data represent average ± SD (error bars) from three independent triplicates; *P < 0.001. (D) Immunoblot analysis of LC3-I and LC3-II levels in HEK293 cells infected for 2 hours with WT Legionella, a dotA mutant, a ∆ravZ mutant, or the complemented RavZ-deficient strain (∆ravZ + pRavZ). (E) HEK293 cells infected for 2 hours with the indicated strains of Legionella were fixed and stained for LC3. Infected cells were scored for the presence of LC3-positive puncta. The graph shows the percent of cells containing LC3-puncta calculated from three independent assays, in which a total of 100 cells were scored in each assay. Data represent the average ± SD; *P < 0.0001 compared to the pentuple mutant control.

An in-frame deletion of the ravZ gene was constructed in a Legionella strain that contained all other effectors. The ∆ravZ mutant and the dotA mutant were equally defective for autophagy inhibition, as measured by LC3-II levels (Fig. 2D) and the presence of LC3 puncta in the infected cells (Fig. 2E). Complementation in both assays was achieved using a plasmid-encoded ravZ allele (Fig. 2, D and E). Likewise, introducing ravZ on a plasmid into the ∆3 strain restored autophagy inhibition during infection, whereas the other nine presumed effectors encoded in the ∆3 region could not (fig. S3). Thus, RavZ is necessary to block autophagy during Legionella infection.

Although AP formation was restored in cells infected with a RavZ-deficient strain of Legionella, LC3-positive membranes were not recruited to vacuoles containing a ∆ravZ mutant (fig. S4). The isogenic ∆ravZ mutant grew equally well in control macrophages and autophagy-deficient macrophages lacking Atg5 when compared to the parental strain (fig. S4). Thus, vacuoles containing the ∆ravZ mutant evade a functional autophagy system, which suggests that Legionella encodes additional effectors capable of disrupting recognition of the vacuole containing Legionella by the autophagy system.

To better understand how RavZ interferes with autophagy, purified RavZ was added to an in vitro assay that reconstitutes conjugation of Atg8 proteins to PE (13). A recombinant human Atg8 homolog called GABARAP-L1 (GR) was conjugated to PE on synthetic liposomes by a reaction requiring adenosine triphosphate and purified Atg7 and Atg3 (Fig. 3A). GR lipidation was strongly inhibited when RavZ was added to this reaction (Fig. 3A). RavZ inhibited GR lipidation when added at concentrations that were more than 1000-fold lower than Atg7 and Atg3 (Fig. 3B), indicating that RavZ functions catalytically to interfere with GR lipidation. Thus, RavZ can inhibit autophagy by acting directly in the Atg8 lipidation pathway.

Fig. 3

RavZ functions as a cysteine protease that specifically deconjugates Atg8 proteins from phospholipid membranes. (A) Coomassie-stained SDS-PAGE gel from an in vitro GR conjugation assay containing the conjugating enzymes Atg7 and Atg3, the RavZ protein, the unconjugated GR protein, and the lipid-conjugated GR protein (GR-PE). Lanes from the whole reaction (rxn) and reactions missing individual components are marked. The RavZ lane contained all the components of the whole reaction plus purified RavZ protein. ATP, adenosine triphosphate. (B) Indicated above each lane are the concentrations of purified RavZ added to a conjugation assay containing GR at a concentration of 12 μM. (C) Liposomes containing GR conjugated to PE were isolated on a flotation gradient (Float-Up lane) and treated with RavZ (+RavZ lane). (D) Liposomes containing GR-PE were treated with RavZ, in the presence of the indicated concentrations of NEM, or with the RavZC258A protein. (E) Fluorescence anisotropy was used to analyze the release kinetics of Texas Red–labeled GR from liposomes in vitro upon treatment with RavZ (red), Atg4B (blue), and RavZC258A (green). (F) Stained SDS-PAGE gels show that GR-YFP was rapidly cleaved by Atg4B to generate the products GR and YFP. No cleavage of GR-YFP was observed by RavZ after a 120-min reaction. (G) LC-MS/MS chromatographs from samples containing purified GR (top) and GR that was isolated after RavZ-mediated deconjugation from PE-containing liposomes (bottom). Arrows indicate that the native C-terminal peptide in GR (DESVYG) was abundant in the untreated sample and that RavZ-mediated deconjugation resulted in a loss of the C-terminal glycine (DESVY). m/z, mass/charge ratio.

The addition of RavZ to isolated liposomes (fig. S5) drove the complete conversion of lipidated GR-PE back to an apparently unmodified form of GR (Fig. 3C). Likewise, direct addition of RavZ to finished in vitro conjugation reactions also resulted in deconjugation of GR-PE (fig. S6). RavZ delipidated all Atg8 family members that we tested (fig. S6). Thus, RavZ is a deconjugating enzyme that targets Atg8 proteins covalently attached to PE.

Atg8 proteins are synthesized as inactive pro-forms that contain additional amino acid residues after the conserved reactive glycine located near the C terminus (5). Atg4 is a cysteine protease that regulates autophagy by cleaving the C terminus of pro-Atg8 after the conserved glycine, which is required for the glycine to participate in the PE conjugation reaction (5). The protease activity of Atg4 can also release Atg8 proteins from vesicles by reversing the amide bond linking the C-terminal glycine to PE (14). Based on their similar ability to deconjugate Atg8 proteins bound to PE, we asked whether RavZ was functioning as an Atg4 mimic.

The addition of N-ethylmaleimide (NEM) to the GR-PE deconjugation reaction, which inhibits cysteine proteases by covalently modifying reactive cysteine residues, interfered with RavZ-mediated deconjugation of GR in vitro (Fig. 3D). Thus, RavZ appears to use a catalytic mechanism similar to Atg4. We used anisotropy to measure the kinetics of deconjugation of fluorescently labeled GR attached to PE on liposome membranes. RavZ deconjugated GR-PE rapidly and Atg4B deconjugation occurred more slowly (Fig. 3E). RavZ deconjugated the entire pool of GR-PE within 1 min, whereas a measureable amount of GR-PE was still detected 60 min after the addition of Atg4B (fig. S7). RavZ did not cleave a recombinant GR protein with yellow fluorescent protein (YFP) fused to the native C terminus (Fig. 3F) or LC3 proteins with extended C-terminal regions (fig. S8), which were all cleaved efficiently by Atg4B in solution. Thus, RavZ and Atg4 have different substrate preferences, with RavZ being highly active on lipid-conjugated Atg8 proteins and having no detectable protease activity toward Atg8 proteins in solution.

We used liquid chromatography–tandem mass spectrometry (LC-MS/MS) to analyze Atg8 reaction products after RavZ treatment. The C-terminal peptide [DESVYG (D, Asp; E, Glu; S, Ser; V, Val; Y, Tyr; G, Gly)] in GR was detected in untreated samples and also in reactions where GR in solution was incubated with RavZ (Fig. 3G). When GR was isolated after being deconjugated from PE by RavZ in vitro, the C-terminal glycine residue had been removed from the peptide (DESVY) (Fig. 3G, bottom graph). Thus, RavZ targeted the amide bond between the tyrosine and the PE-conjugated glycine residue, unlike Atg4, which targets the amide bond linking glycine to PE (fig. S9). Thus, RavZ is a deconjugating enzyme that produces an Atg8 product that would be resistant to reconjugation by the host machinery due to the absence of the reactive C-terminal glycine.

The otubain family of deubiquitinating enzymes are proteases with a reactive cysteine residue presented in a conserved DGNC (N, Asn; C, Cys) sequence (15), which closely matched the cysteine residue at amino acid position 258 in RavZ that is presented in the context of an EGNC motif. RavZC258A derivatives were inactive in the in vitro assays that measure GR-PE deconjugation (Fig. 3D), the in vivo assays that measure LC3-II conversion and AP formation by staining LC3 puncta (fig. S10), and the starvation-mediated long-lived protein degradation assay (Fig. 2D). GFP-RavZ localized to structures that were Atg16-positive and LC3-negative, whereas GFP-RavZC258A localized structures that were Atg16-negative and LC3-positive (fig. S11). Thus, RavZ can bind early AP membranes, and RavZC258A represents a noncatalytic substrate-trapping mutant that remains associated with Atg8-PE on mature AP membranes.

We have found that the Legionella RavZ protein inhibits host autophagy by functioning as a cysteine protease that specifically targets lipid-conjugated Atg8 proteins and generates a deconjugated Atg8 product that lacks the essential C-terminal glycine required for reconjugation. RavZ can both prevent Atg8 from accumulating on the membranes of phagophores that mature into AP structures and also inactivate the Atg8 protein during the deconjugation reaction. Thus, Legionella has evolved a specific mechanism to interfere with host autophagy by directly targeting the Atg8 proteins involved in AP formation.

Supplementary Materials

www.sciencemag.org/cgi/content/full/science.1227026/DC1

Materials and Methods

Figs. S1 to S11

References (1625)

References and Notes

  1. Acknowledgments: We thank N. Mizushima and A. Iwasaki for providing GFP-LC3 and Atg5-deficient mice and S. Shin for providing the plasmid encoding BlaM-RalF. Support for this research came from the Science, Technology and Research Scholars Program at Yale University (B.M.); the Howard Hughes Medical Institute (T.J.O. and R.R.I.); and NIH Awards AI007019 (A.C.), NS063973 (T.J.M.), AI041699, and AI048770 (C.R.R.). The data presented in this manuscript are tabulated in the main paper and in the supplementary materials.
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