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GPR15-Mediated Homing Controls Immune Homeostasis in the Large Intestine Mucosa

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Science  21 Jun 2013:
Vol. 340, Issue 6139, pp. 1456-1459
DOI: 10.1126/science.1237013

GPR15 Gets Tregs to Guard the Gut

The large intestine is the site that is typically most inflamed in Crohn's disease and ulcerative colitis, which are thought to result when the immune system is not able to keep the peace with trillions of resident gut microbes. The immune system does this by recruiting specific cell populations, like regulatory T cells (Tregs), to the gut. Kim et al. (p. 1456, published online 9 May) now suggest that the orphan G protein–coupled receptor GPR15 is expressed by Tregs and required for Treg homing to the large intestine in mice.

Abstract

Lymphocyte homing, which contributes to inflammation, has been studied extensively in the small intestine, but there is little known about homing to the large intestine, the site most commonly affected in inflammatory bowel disease. GPR15, an orphan heterotrimeric guanine nucleotide–binding protein (G protein)–coupled receptor, controlled the specific homing of T cells, particularly FOXP3+ regulatory T cells (Tregs), to the large intestine lamina propria (LILP). GPR15 expression was modulated by gut microbiota and transforming growth factor–β1, but not by retinoic acid. GPR15-deficient mice were prone to develop more severe large intestine inflammation, which was rescued by the transfer of GPR15-sufficient Tregs. Our findings thus describe a T cell–homing receptor for LILP and indicate that GPR15 plays a role in mucosal immune tolerance largely by regulating the influx of Tregs.

The microbiota of the human gut have coevolved with the host (13), and their coexistence is due in large part to an equilibrium established with the host immune system (4). In the gastrointestinal tract, the large intestine harbors significantly more microbiota than the small intestine (5) and contains higher frequencies of FOXP3+ regulatory T cells (Tregs) (68). Disruption of the equilibrium between the host immune system and microbiota can trigger inflammatory bowel disease (IBD) in mouse models and, in humans, likely contributes to Crohn’s disease and ulcerative colitis (9), in which the large intestine is the primary site of inflammation. Although T cell responses have critical roles in IBDs (9), it remains unclear how T cells migrate to the large intestine (1012). Retinoic acid (RA) regulates lymphocyte migration to the small intestine but not to the large intestine (10, 11), which indicates that there is a separate mechanism for this process.

Human GPR15 (also known as BOB) was originally cloned as a co-receptor for HIV or the simian immunodeficiency virus (13, 14). To study the physiological function of its murine ortholog, we made knock-in mice in which endogenous Gpr15 was replaced with the sequence for green fluorescent protein (GFP) (fig. S1). In humans, GPR15 mRNA is highly expressed in the colon, peripheral blood lymphocytes (PBLs), and spleen (13). Similarly, in mice, GFP expression was detected in gut tissues and lymphoid organs, where it was largely restricted to T cell receptor β–positive (TCRβ+) cells (fig. S2, A and B). T cells in the large intestine lamina propria (LILP) exhibited the highest percentage of GFP+ cells, whereas GPR15 expression was minimal in other immune system cells in the LILP (fig. S2, C to F). To determine the functional characteristics of GPR15+ cells, we analyzed the transcriptomes of GFP and GFP+ CD4+ T cells from the LILP by microarray (table S1). Many of the genes highly expressed in GFP+ cells, compared with GFP cells, were characteristic of FOXP3+ Tregs [Foxp3 (15), Eos (16), interleukin-10 (Il10) (17), and Cd25 (18)] (table S1). We confirmed the preferential expression of GPR15 in Tregs by analyzing Foxp3 reporter expression in Gpr15gfp/+ Foxp3ires-mrfp mice (19) (Fig. 1A) and also by staining for FOXP3 protein (fig. S2, G and H). About 60 to 70% of LILP CD4+FOXP3+ cells expressed Gpr15, compared with only 7 to 20% of CD4+FOXP3 cells, in mice of two different genetic backgrounds (Fig. 1A and fig. S2H).

Fig. 1 GPR15 is preferentially expressed in and regulates the frequency of FOXP3+ regulatory T cells in the LILP.

(A) Gpr15gfp/+ mice were bred to Foxp3ires-mrfp mice. GFP and monomeric red fluorescent protein (mRFP) expression was examined in T cell subsets from different tissues (DN T: CD4CD8β T cells). FSC-A, (forward-scatter). Results shown are representative of at least three independent experiments. (B) Percentage of FOXP3+ Tregs among CD4+ T cells in different tissues of Gpr15gfp/+ mice (Het) and Gpr15gfp/gfp (KO) mice [B6N10 (C57BL/6-backcrossed 10 times): n = 9; combined from at least two independent experiments]. (C) Numbers of FOXP3+ (left) and FOXP3 cells (right) in the LILP were compared between OT-II Rag2–/– Gpr15gfp/+ (Het) and OT-II Rag2–/– Gpr15gfp/gfp (KO) mice after OVA administration (n = 12, combined from four independent experiments). *P < 0.05 (t test).

We next determined whether disproportionate expression of GPR15 in Tregs could affect their presence in the gut. We observed a reduction in the Treg percentage in the LILP but not in the small intestine lamina propria (SILP) or spleen of Gpr15 knockout (KO) compared with Gpr15 heterozygous (Het) mice (Fig. 1B and fig. S3A). Both thymus-derived and peripherally derived Tregs were equally affected (fig. S3B). In cell numbers, Tregs, CD8+ T cells, and double-negative (DN) T cells, all of which showed significant GPR15-GFP expression, were reduced in the LILP of Gpr15 KO mice (fig. S3C). These populations were unaffected in the SILP (fig. S3D). There was a significant, but much smaller, reduction in CD4+FOXP3 T cells (fig. S3C), such that there was an overall decrease in Treg percentage among total CD4+ T cells in the LILP (Fig. 1B and fig. S3A).

We next examined Treg frequency in the LILP during an antigen-specific T cell response. Rag2–/–, OT-II TCR transgenic mice that were heterozygous or homozygous for the Gpr15gfp allele were fed with chicken ovalbumin (OVA). Without antigen exposure, all T cells maintained a naïve phenotype (CD44lo), and no Treg or GFP+ T cells were observed (fig. S4A). After OVA exposure of heterozygous mice, there was a small influx in the LILP of GFP+ T cells (2 to 5%) (fig. S4A) that were enriched for FOXP3 expression (fig. S4B). There was a significant reduction in the number and frequency of Tregs but not in the number of CD4+FOXP3 T cells in the LILP of KO mice (Fig. 1C and fig. S4C). Thus, GPR15 preferentially contributes to Treg frequency in the LILP at steady state and during an antigen-specific T cell response.

To determine whether GPR15 functions as a homing receptor for the LILP, we performed a short-term competitive homing assay by co-injecting T cells transduced with a control or a GPR15-encoding retrovirus into congenic hosts (fig. S5A). When GPR15+ cells and control cells were mixed at a 1:1 ratio and transferred into C57BL/6 mice, all tissues examined exhibited a 1:1 ratio of the donor-derived cells, except for the LILP, where there was a ~10-fold enrichment for GPR15+ cells (Fig. 2A and fig. S5B). There was minimal homing of transferred cells to the small intestine (fig. S5B). When GPR15+ cells were treated with the Gαi inhibitor pertussis toxin (PTX) before transfer, they were no longer enriched in the LILP (Fig. 2B), which indicated that GPR15 likely signals through Gαi, as do other lymphocyte homing receptors. Many G protein–coupled receptors (GPCRs) have, in their second intracellular loop, a conserved DRY motif that is important for downstream signaling through its interactions with heterotrimeric G proteins (20). To ensure that active signaling through GPR15 was required for homing, we mutated the GPR15 DRY motif to DAY [Arg131 replaced by Ala (R131A)]. Although both wild-type (WT) and mutant proteins were similarly expressed at the cell surface (fig. S5C), only cells expressing the WT fusion protein migrated to the LILP (Fig. 2C and fig. S5D).

Fig. 2 GPR15 mediates T cell homing to the LILP.

(A) Ratio of Gpr15-transduced and control-transduced donor cells in different tissues (MLN: mesenteric lymph nodes; PLN: inguinal, brachial, and axillary lymph nodes) 10 hours after transfer of an equal number of cells (n = 6, combined from three independent experiments). (B) Ratio of Gpr15-transduced cells treated with PTX and untreated control-transduced cells after cotransfer (n = 5). (C) Ratio of cells transduced with control vector and the R131A mutant Gpr15 fused with gfp (GPR15mut-GFP) (n = 7, combined from three independent experiments). (D) Ratio in different tissues of CD4+ T cells from Gpr15gfp/+(Het) and Gpr15gfp/gfp (KO) mice after in vitro culture in GPR15-inducing conditions and transfer of equal numbers of cells into recipients (n = 5, combined from two independent experiments). *P < 0.05 (t test).

Preferential homing of GPR15+ cells to the LILP was observed as early as 2 hours after cell transfer, which suggested that activation of this GPCR may promote integrin-dependent interaction of T cells with the endothelium in the target organ (fig. S5E). Indeed, blocking antibodies against either subunit of the α4β7 integrin inhibited GPR15-mediated homing to the LILP (fig. S5F). Unlike α4β7 and CCR9 (21), GPR15 was not induced by RA (fig. S6). However, GPR15 was induced in T cells treated with a combination of transforming growth factor–β1 (TGF-β1) and either IL-6 or IL-21 (fig. S7), and there was a marked decrease in Gpr15 mRNA in T cells of Tgfb1C33S/C33S mice that have reduced TGF-β1 in vivo (22) (fig. S8, A and B). In contrast, Il21r–/–Il6–/– mice crossed with Gpr15gfp/+ mice had a similar level of GFP expression as control mice (fig. S8C), which suggested that only TGF-β1 is a key regulator of GPR15 expression in vivo. Cells from Gpr15 Het and KO mice were treated with these cytokines to induce GPR15 expression in vitro and were used in the short-term competitive homing assay (Fig. 2D). The results confirmed the importance of endogenously expressed GPR15 in the homing of T cells to the LILP.

We also tested the effect of gut microbiota on GPR15-mediated homing of T cells to the LILP. Treatment of Gpr15gfp/+ mice with a combination of broad-spectrum antibiotics led to a decrease in GPR15 expression (fig. S9A). In contrast, GPR15-overexpressing T cells preferentially migrated to the LILP even in germ-free or antibiotics-treated recipients (fig. S9, B and C). Therefore, microbiota can affect GPR15 expression but are unlikely to produce ligand(s) for GPR15.

Because GPR15 deficiency affected Treg homing to the LILP, we next investigated its role in immune homeostasis in the large intestine. We first examined cytokine production by CD4+ T cells in the large intestines of Gpr15 Het and KO mice. At steady state, there was an increased proportion of interferon (IFN)-γ– and IL-17A–producing cells among total CD4+ T cells in the LILP of Gpr15 KO mice on a 129/B6 mixed background (fig. S10A) but not in C57BL/6-backcrossed mice. However, when C57BL/6-backcrossed mice were injected with CD40 antibody [which induces acute colitis in Rag2–/– mice (23)], inflammatory cytokine expression in the large intestine (but not spleen) was higher in Gpr15 KO mice than in littermate controls (fig. S10, B and C). We next tested the physiological consequences of this inflammatory phenotype in an infection-induced colitis model. When mice were infected with Citrobacter rodentium, the majority of WT mice resolved inflammation and survived. In contrast, most Gpr15 KO mice suffered severe weight loss and died (Fig. 3, A and B). KO mice also exhibited increased inflammation, tissue damage, and inflammatory cytokine expression (Fig. 3, C and D, and fig. S10D), and Treg numbers in the LILP were reduced compared with Het mice (fig. S10E). The large intestine and spleen of Het and KO mice had a similar pathogen load (fig. S10F), which indicated that GPR15 is not required for controlling the infection but rather for dampening the immune response in the large intestine.

Fig. 3 GPR15-deficient mice are prone to inflammation of the large intestine because of a defect in Tregs.

(A to F) Results after infection of mice with C. rodentium. (A) Kaplan-Meier survival curve of WT and KO mice (WT: n = 9; KO: n = 22, combined from three independent experiments). (B) Weight change (n = 6 to 8, representative of three independent experiments). (C and E) Hematoxylin and eosin (H&E) staining of colon sections (scale bar, 70 μm) of C. rodentium–infected Het and KO mice (C) or of chimeric mice reconstituted with bone marrow progenitors for GPR15-sufficient (WT) or GPR15-deficient (KO) Tregs (E). (D and F) Histology score and inflammation index of colons of Het and KO mice (D) or of mixed–bone marrow chimeras (F) (n = 5 to 6 per group). *P < 0.05 (t test), **P < 0.05 (log-rank test).

To confirm that sensitivity to Citrobacter infection was due to a role of GPR15 in Tregs rather than other T cells, we infected lethally irradiated mice that received mixed bone marrow from Foxp3sf and from either WT or Gpr15 KO mice. In Foxp3sf + Gpr15 KO mixed chimeras, Tregs will develop only from Gpr15 KO bone marrow and will thus lack GPR15 expression, whereas other T cells can develop from GPR15-sufficient Foxp3sf bone marrow. Indeed, Foxp3sf + Gpr15 KO mixed chimeric mice exhibited more severe inflammation and tissue damage than did chimeras generated with WT bone marrow (Fig. 3, E and F), which indicated that GPR15 expression in Tregs is required to prevent severe colitis following Citrobacter infection. This phenotype was not due to a role of GPR15 in regulating Treg function, as WT Tregs and KO Tregs suppressed naïve T cell proliferation equally well (fig. S11A). These results indicate that GPR15 is critical for preventing pathological inflammation in the large intestine during colitis, most likely by regulating Treg homing.

We next used a noninfectious model of colitis to determine the role of GPR15 in suppressing local inflammation in vivo. CD40 stimulation in the absence of adaptive immunity induces innate immune cell–mediated colitis (23) that can be rescued by introduction of Tregs (24, 25). We therefore transferred Tregs from Gpr15 WT or KO mice into Rag2–/– mice that were subsequently treated with CD40 antibody. The transfer of WT Tregs, but not KO Tregs, reduced colitis severity and tissue damage (Fig. 4, A and B). We also determined the ability of naïve T cells from Gpr15 WT and KO mice to induce colitis after Helicobacter hepaticus infection (24). In this T cell–transfer colitis model, which is dependent on the absence of Tregs, KO Tnaïve cells induced colitis as well as their WT counterpart (fig. S11B), consistent with a preferential role for GPR15 in regulating the homing of Tregs.

Fig. 4 Tregs from GPR15-deficient mice cannot rescue colitis.

(A and B) Rag2–/– mice received 5 × 105 mRFP+ Tregs transferred from either Foxp3ires-mrfp or Gpr15gfp/gfp Foxp3ires-mrfp mice that were subsequently injected with CD40 antibody (FGK45). (A) H&E staining of proximal and midcolon section of mice without any treatment, with colitis induction alone, or with colitis induction and rescue by WT or KO Tregs (scale bar, 70 μm). (B) Histology scores (n = 7 to 12, combined from two independent experiments). *P < 0.05 (t test).

To determine whether the function of GPR15 is conserved between human and mouse, we examined GPR15 mRNA expression in different cell types from various human tissues. Similar to GPR15 expression in mice, that in humans was minimal in lymphocytes from the blood and the small intestine; however, it was expressed at high levels in lymphocytes from the large intestine (fig. S12). In contrast, we did not detect elevated GPR15 mRNA expression in the Treg-enriched CD25+CD4+ T cell population relative to other LILP T cell populations. Rather, there was more GPR15 mRNA in CD25CD4+ T cells than in CD25+CD4+ T cells. All colon samples were from colorectal carcinoma patients; thus, the pattern of GPR15 expression in colonic lymphocytes from normal subjects, IBD patients, and patients with HIV-mediated enteropathy (26, 27) remains to be determined.

Our results indicate that the small and the large intestine use different homing cues and different homing receptors for adaptive immune cells (fig. S13A) and, thereby, compartmentalize immune tolerance mediated by Tregs (fig. S13B). GPR83 is also preferentially expressed in Tregs and has been suggested to contribute to Treg cell differentiation in inflammatory conditions (28). We cannot rule out a similar role for GPR15, in addition to its function in directing migration or retention of T cells in the large intestine. It will thus be interesting to examine the role of other inflammatory signals in GPR15-dependent Treg function. Our results improve understanding of immune homeostasis in the intestinal mucosa and could potentially lead to new therapeutic strategies to treat inflammatory diseases by combining in vitro expansion of Tregs and GPR15 induction for reintroduction into patients.

Supplementary Materials

www.sciencemag.org/cgi/content/full/science.1237013/DC1

Materials and Methods

Figs. S1 to S13

Table S1

References (2936)

References and Notes

  1. Acknowledgments: The authors thank R. Min and P. Oh for technical assistance; M. Sellars for a critical reading of the manuscript; J. M. Weiss, J. J. Lafaille, and A. Y. Rudensky for their advice; and E. Newman (NYU School of Medicine, New York) for providing surgical specimens. Foxp3ires-mrfp mice were provided by R. A. Flavell (Yale University School of Medicine, Howard Hughes Medical Institute) and subject to a Materials Transfer Agreement. A patent application related to the work in this paper has been filed (S.V.K. and D.R.L. as inventors). The data in this paper are tabulated in the main paper and in the supplementary materials, and are deposited in National Center for Biotechnology Information’s Gene Expression Omnibus (GSE45773). S.V.K. was supported by the Irvington Institute fellowship program of the Cancer Research Institute and by NIH National Research Service Award grant 1T32AI100853-01. Y.Y. was supported by the Arthritis National Research Foundation. D.R.L. is a Howard Hughes Medical Institute Investigator. This work was supported in part by National Cancer Institute (NCI), NIH, grant 5P30CA016087-32 (BioRepository Center, Genome Technology Center, and Histopathology Core), NCI-NIH grant 5P30CA016087-33 (Cytometry and Cell Sorting Core), National Center for Research Resources, NIH, grant UL1RR029893 (BioRepository Center), and NCI-NIH P30 CA016087-30 (for data collected on the Affymetrix GeneChip System).
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