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Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

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Science  03 Jan 2014:
Vol. 343, Issue 6166, pp. 84-87
DOI: 10.1126/science.1247005

Article Information

vol. 343 no. 6166 84-87

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History: 
  • Received for publication October 9, 2013
  • Accepted for publication December 2, 2013

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Author Information

  1. Ophir Shalem1,2,*,
  2. Neville E. Sanjana1,2,*,
  3. Ella Hartenian1,
  4. Xi Shi1,3,
  5. David A. Scott1,2,
  6. Tarjei S. Mikkelsen1,
  7. Dirk Heckl4,
  8. Benjamin L. Ebert4,
  9. David E. Root1,
  10. John G. Doench1,
  11. Feng Zhang1,2,
  1. 1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA.
  2. 2McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
  3. 3Stanley Center for Psychiatric Research, Broad Institute of Harvard and MIT, 7 Cambridge Center, Cambridge, MA 02142, USA.
  4. 4Division of Hematology, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA 02115, USA.
  1. Corresponding author. E-mail: zhang{at}broadinstitute.org
  1. * These authors contributed equally to this work.

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