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Science  23 Jan 2015:
Vol. 347, Issue 6220, pp. 443
DOI: 10.1126/science.347.6220.443-a

Multiblock PCR Thermal Cycler

The new Mastercycler nexus X2 is ideal for researchers looking to carry out two polymerase chain reactions (PCR) simultaneously, without any compromise on the number of samples. The instrument comprises of two asymmetric blocks, consisting of 64 and 32 wells, which can be programmed and run completely independently, enabling two separate PCR protocols to be run in parallel. With reduced noise emission (<40 dB), low power consumption and a small, well-designed footprint, the Mastercycler nexus X2 is perfectly suited for use in busy academic laboratories, or any institution with multiple users. In comparison to other dual block cyclers, the Mastercycler nexus X2 provides an elegant solution for users wishing to run procedures using a large number of samples, without taking up a large amount of bench space. As the latest addition to the popular Mastercycler range, the Mastercycler nexus X2 continues Eppendorf's legacy of exceptional design and ease of use combined with efficiency and accuracy.

RNA/Protein Detection Assay

The PrimeFlow RNA Assay is the first and only flow cytometry assay capable of simultaneous detection of RNA and protein within millions of cells at single-cell resolution. With the novel PrimeFlow assay, researchers can now incorporate the simultaneous analysis of RNA transcripts and proteins to elevate their understanding of single-cell dynamics. PrimeFlow RNA Assay enables high throughput detection of RNA and protein expression; therefore, it can be used to characterize, mechanistically and phenotypically, co-expression of RNAs with the functional proteins at the single-cell level. This assay, with a user-friendly protocol that has many similarities with standard antibody-staining procedures and data acquisition of flow cytometry, is an invaluable tool for immunology labs performing translational research. The major advantage of this technology is the ability to detect, with high sensitivity, mRNAs for which flow cytometry antibodies against the corresponding proteins perform poorly or are not available.

Gene Engineering System

The Dharmacon Edit-R Gene Engineering System is a novel platform for research which creates permanent and heritable gene disruption (knockouts) in cells utilizing the CRISPR-Cas9 gene engineering technology. Harnessing the natural defense mechanism of S. Pyogenes, the Edit-R platform comprises the three components required for gene editing in mammalian cells: (1) A plasmid encoding Cas9 nuclease, (2) a chemically synthesized trans-activating RNA (tracRNA), and (3) a custom CRISPR RNA (crRNA) designed to target the site of interest. The Edit-R platform greatly simplifies and accelerates the experimental requirements and workflow compared to existing approaches. Through easy co-transfection of synthetic tracrRNA and crRNAs using DharmaFECT Duo transfection reagent, researchers can program the Cas9 nuclease to target their genes of interest without the need to clone and sequence individual guide RNAs into expression vectors. The simplified workflow for this powerful reverse genetics method can generate functional knockouts in one to two weeks versus the current one-month timescale.

Multiplex Amplicon Panels

An early access program is now available for Accel-NGS Amplicon Panels for use with Illumina Next Generation Sequencing (NGS) systems. The assays are compatible with FFPE and circulating cell free DNA from 10 ng of genomic DNA. The underlying technology enables hundreds of primer pairs to be amplified in a single tube. The panels can be used to target either contiguous coverage of a single gene, multiple loci throughout the genome, or a combination of both. The initial product is a comprehensive TP53 panel, a clinically important tumor suppressor gene. This panel covers all coding exons of TP53 in a single tube format. Another panel under development based on this technology targets over 200 clinically relevant oncology mutations. The multiplex amplicon panels achieve high-performance metrics, such as percent on-target and coverage uniformity. This new technology has a rapid workflow allowing identification of important mutations in a cost-effective manner.

Gene Capture Probes

xGen Predesigned Gene Capture Pools and Plates are ideal for creating customized target capture panels for enrichment of 10 or more genes or for enhancing the performance of existing panels. Predesigned Gene Capture Pools and Plates are ideal for scientists who need to reduce the cost of their next generation sequencing (NGS) experiments without sacrificing quality and specificity. xGen Predesigned Gene Capture Pools are available for the coding regions of any human RefSeq gene and are delivered premixed in tubes or in individual plate wells for selective mixing. NGS target capture technologies have previously been available mostly as either fixed, off-the-shelf probe pools, or panels that do not always meet experimental needs or expensive custom panels. With xGen Predesigned Gene Capture Pools and Plates, researchers and core labs can now generate relevant, custom gene panels in-house. This customization allows smaller starting volumes per panel, resulting in less waste.

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