Report

Genetic control of distal stem cell fate within root and embryonic meristems

See allHide authors and affiliations

Science  06 Feb 2015:
Vol. 347, Issue 6222, pp. 655-659
DOI: 10.1126/science.aaa0196
  • Fig. 1 Phenotype of the nww mutant and expression of NWW genes in embryos.

    (A) Stages in root meristem development, from an early globular-stage embryo to a mature root meristem with different regions of the meristem labeled and colored. QC, quiescent center. (B and C) Seedling phenotype of the wild type (B) and nww mutant (C). The arrow in (C) indicates the absence of the root. (D to G) Arrows indicate root meristem initiation in globular (glob) and heart-stage embryos of the wild type [(D) and (F)] and nww mutant [(E) and (G)]. (H to K) Recombineered NTT [(H) and (I)], WIP4 (J), and WIP5 (K) reporters in a 32-cell or early globular stage before root meristem initiation [arrows in (H), (J), and (K)] and a late globular stage (I). Arrows point to hypophysis (hypo) cell in (H), (J), and (K). Scale bars, 20 μm in (D) to (K).

  • Fig. 2 Expression analysis in WT and mutant embryos.

    (A and B) Arrows indicate gNTT-n2YPET accumulation in divided hypophysis of WT (A) and mp mutant (B) globular-stage embryos. (C and D) In situ hybridization of MP in globular-stage embryos. To ensure that we were not detecting background signal, we used a segregating mp mutant population along with an in situ probe that only detected WT MP transcript. Arrows show expression in outlined hypophysis (C) of early globular-stage embryo, but no expression in a phenotypically mp mutant (D) late globular-stage embryo. (E) Accumulation of recombineered gMP-n2YPET throughout an early globular embryo. The arrow indicates accumulation within the hypophysis. (F) Diagram of the genetic pathway to promote root initiation. (G and H) Normal initiation of pDR5-GFP expression is indicated by arrows in the wild type (G) and nww mutant (H). (I and J) Normal initiation of pWOX5-GFP expression indicated by arrows in the wild type (I) and nww mutant (J). Scale bars, 20 μm in (A) to (E) and (G) to (J).

  • Fig. 3 Control of distal meristem fate within the root.

    (A and B) Accumulation of NTT (A) and WIP4 (B) in the quiescent center and columella initials (arrow) of the root meristem. (C and D) Rescued root phenotype of the wild type (C) and nww mutant (D) after auxin treatment and 2 days after transfer to MS media. (E to L) Phenotype of the wild type [(E), (G), (I), and (K)] and nww mutant [(F), (H), (J), and (L)]. The QC25 marker is indicated by an arrow in the wild type (E) and is absent in nww mutant (F). pPIN7-PIN7-GFP marker, with distal expression indicated by an arrow in the wild type (G) and absent in the nww mutant (H). pWOX5-GFP [(I) and (J)] and pDR5-GFP [(K) and (L)] markers in the wild type [(I) and (K)] and nww mutant [(J) and (L)]. Scale bars, 20 μm in (A), (B), and (E) to (L); 1 mm in (C) and (D).

  • Fig. 4 Analyses of NTT misexpression.

    (A and B) Root meristem of p35S-NTT-GR uninduced (A) and induced (B) roots stained for amyloplasts (brackets show WT staining) and with the QC25 marker with blue staining. (C and D) Seedlings of pAS1-LHG4 driver line control (C) and pAS1>>NTT (D). (E and F) Torpedo stage of WT (E) and pML1>>NTT (F) embryos with pDR5-GFP marker. Scale bars, 20 μm in (A), (B), (E), and (F).

Supplementary Materials

  • Genetic control of distal stem cell fate within root and embryonic meristems

    Brian C. W. Crawford, Jared Sewell, Greg Golembeski, Carmel Roshan, Jeff A. Long, Martin F. Yanofsky

    Materials/Methods, Supplementary Text, Tables, Figures, and/or References

    Download Supplement
    • Materials and Methods
    • Figs. S1 to S8
    • Table S1
    • Full Reference List

Stay Connected to Science

Navigate This Article