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The mutagenic chain reaction: A method for converting heterozygous to homozygous mutations

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Science  24 Apr 2015:
Vol. 348, Issue 6233, pp. 442-444
DOI: 10.1126/science.aaa5945

Generating homozygous mutations

Loss-of-function mutations may only produce a mutant phenotype when both copies of the gene are mutated. Gantz and Bier developed a method they call mutagenic chain reaction (MCR) that autocatalytically produces homozygous mutations. MCR uses the initial mutated allele to cause a mutation in the allele on the opposing chromosome and thus the homozygosity of the trait. MCR technology could have broad applications in diverse organisms.

Science, this issue p. 442