Research Article

The 3.8 Å structure of the U4/U6.U5 tri-snRNP: Insights into spliceosome assembly and catalysis

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Science  29 Jan 2016:
Vol. 351, Issue 6272, pp. 466-475
DOI: 10.1126/science.aad6466

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Structure of a key spliceosomal complex

In eukaryotes, when DNA is transcribed into RNA, the primary transcript has protein-coding sequences interrupted by noncoding sequences called introns. Introns are removed by a complex molecular machine, the spliceosome. Wan et al. determined the structure of a key subcomplex, the tri-SNP, that comprises three small nuclear RNAs and more than 30 proteins. The structure, determined by electron microscopy at 3.8 Å resolution, unexpectedly shows a primary RNA transcript bound in the tri-SNP. Further analysis revealed how the spliceosome assembles to achieve its complex functions.

Science, this issue p. 466

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